Genome-wide reconstitution of dynamics in nucleosome positioning, collision, eviction, and histone exchange (A04)

• Subject Area: General Genetics and Functional Genome Biology
• Term: since 2013
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 213249687

We use a unique genome-wide chromatin reconstitution system with purified factors to explore remodeler mechanisms of nucleosome positioning, collision, eviction, generation of subnucleosomal particles and histone exchange, at steady state and during kinetics. The genome-wide approach allows direct comparisons with corresponding in vivo data. With Hopfner (A06) we will include structure-guided yeast INO80 mutant remodelers and human INO80. Probing the role of genomic DNA sequence, barriers, histone modifications/variants and histone chaperones will reveal how remodelers process multiple levels of information into remodeler-specific outcomes.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1064: Chromatin Dynamics
• Applicant Institution: Ludwig-Maximilians-Universität München
• Project Head: Professor Dr. Philipp Korber