Dynamic respiratory regulation in plant mitochondria

• Applicant: Professor Dr. Markus Schwarzländer
• Subject Area: Plant Physiology
• Term: from 2013 to 2020
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 237658134

• Final Report Year: 2020

Final Report Abstract

Overall, the Emmy-Noether group funded through this grant made significant progress on the proposed project. In several instances we could go beyond the level of insight that we had been hoping for; in cases where we faced problems we have been able to find pragmatic solutions. We have gained a number of fundamental and exciting new insights into how plant mitochondria work and how they impact on plant cell physiology. In addition, we made several methodological advances on the way. The project has opened up new lines of research that we and others have started following up actively. Further follow up will need to be organized based on separate funding and I am currently preparing two additional funding applications that are directly based on the data from this project.

Publications

• (2014) The ‘mitoflash’ probe cpYFP does not respond to superoxide. Nature. 514 (7523): E12-E14
  Schwarzländer M, Wagner S, Ermakova YG, Belousov VV, Radi R, Beckman JS, Buettner GR, Demaurex N, Duchen MR, Forman HJ, Fricker MD, Gems D, Halestrap AP, Halliwell B, Jakob U, Johnston IG, Jones NS, Logan DC, Morgan B, Müller FL, Nicholls DG, Remington SJ, Schumacker PT, Winterbourn CC, Sweetlove LJ, Meyer AJ, Dick TP, Murphy MP
  (See online at https://doi.org/10.1038/nature13858)
• (2015) The EF-hand Ca2+-binding protein MICU choreographs mitochondrial Ca2+ dynamics in Arabidopsis. Plant Cell. 27 (11): 3190-3212
  Wagner S, Behera S, De Bortoli S, Logan DC, Fuchs P, Carraretto L, Teardo E, Cendron L, Nietzel T, Füßl M, Doccula FG, Navazio L, Fricker MD, Van Aken O, Finkemeier I, Meyer AJ, Szabò I, Costa A, Schwarzländer M
  (See online at https://doi.org/10.1105/tpc.15.00509)
• (2015) The mitochondrial monothiol glutaredoxin S15 is essential for maturation of iron-sulfur proteins in Arabidopsis thaliana. Proc Natl Acad Sci USA. 112 (44): 13735-13740
  Moseler A, Aller I, Wagner S, Nietzel T, Przybyla-Toscano J, Mühlenhoff U, Lill R, Berndt C, Rouhier N, Schwarzländer M, Meyer AJ
  (See online at https://doi.org/10.1073/pnas.1510835112)
• (2017) ATP sensing in living plant cells reveals tissue gradients and stress dynamics of energy physiology. eLIFE. e26770
  De Col V, Fuchs P, Nietzel T, Elsässer M, Voon CP, Candeo A, Seeliger I, Fricker MD, Grefen C, Møller IM, Bassi A, Lim BL, Zancani M, Meyer AJ, Costa A, Wagner S, Schwarzländer M
  (See online at https://doi.org/10.7554/eLife.26770)
• (2017) Physiological characterization of a plant mitochondrial calcium uniporter in vitro and in vivo. Plant Physiol. 173(2): 1355-1370
  Teardo E, Carraretto L, Wagner S, Formentina E, Behera S, De Bortoli S, Larosa V, Fuchs P, Lo Schiavo F, Raffaello A, Rizzuto R, Costa A, Schwarzländer M, Szabò I
  (See online at https://doi.org/10.1104/pp.16.01359)
• (2018) ATP compartmentation in plastids and cytosol of Arabidopsis thaliana revealed by fluorescent protein sensing. Proc Natl Acad Sci USA. 115 (45) e10778-e10787
  Voon CP, Guan X, Sun Y, Sahu A, Chan MN, Gardeström P, Wagner S, Fuchs P, Nietzel T, Versaw WK, Schwarzländer M, Lim BL
  (See online at https://doi.org/10.1073/pnas.1711497115)
• (2018) Cellular Ca2+ signals generate defined pH signatures in plants. Plant Cell. 30(11): 2704-2719
  Behera S, Xu Z, Luoni L, Bonza C, Doccula FG, DeMichelis MI, Morris RJ, Schwarzländer M, Costa A
  (See online at https://doi.org/10.1105/tpc.18.00655)
• (2019) Multiparametric real-time sensing of cytosolic physiology links hypoxia responses to mitochondrial electron transport. New Phytol. 224(4): 1668-1684
  Wagner S, Steinbeck J, Fuchs P, Lichtenauer S, Elsässer M, Schippers JHM, Nietzel T, Ruberti C, Van Aken O, Meyer AJ, Van Dongen JT, Schmidt RR, Schwarzländer M
  (See online at https://doi.org/10.1111/nph.16093)
• (2019) The fluorescent protein sensor roGFP2-Orp1 monitors in vivo H2O2 and thiol redox integration and elucidates intracellular H2O2 dynamics during elicitor-induced oxidative burst in Arabidopsis. New Phytol. 221(3): 1649-1664
  Nietzel T, Elsässer M, Ruberti C, Steinbeck MJ, Ugalde JM, Fuchs P, Wagner S, Ostermann L, Moseler A, Lemke P, Fricker MD, Müller-Schüssele SJ, Moerschbacher BM, Costa A, Meyer AJ, Schwarzländer M
  (See online at https://doi.org/10.1111/nph.15550)
• (2020) Redox-mediated kick-start of mitochondrial energy metabolism drives resource-efficient seed germination. Proc Natl Acad Sci USA
  Nietzel T, Mostertz J, Ruberti C, Née G, Fuchs P, Wagner S, Moseler A, Müller-Schüssele SJ, Benamar A, Poschet G, Büttner M, Møller IM, Lillig CH, Macherel D, Wirtz M, Hell R, Finkemeier I, Meyer AJ, Hochgräfe F, Schwarzländer M
  (See online at https://doi.org/10.1073/pnas.1910501117)