DNA assemblies for structural analysis of guest molecules and plasmon-enhanced SERS (A06)

• Subject Area: Statistical Physics, Nonlinear Dynamics, Complex Systems, Soft and Fluid Matter, Biological Physics
• Term: from 2012 to 2024
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 201269156

In the first part of the project DNA origami-based crystal growth will be optimised to achieve single-crystals of tens of micrometers, preferably up to 100 micrometer. DNA origami crystals will then be used for structural analysis of incorporated guest molecules (e.g. proteins) using small angle X-ray scattering (SAXS, nano-SAXS and free electron lasers (FEL)). We will make use of our ability to silicify our DNA structures to increase their thermal and mechanical stability.In the second part of this project we will place metallic nanoparticles and nanorods with nanometer precision on DNA origami scaffolds. These antenna complexes will be used for SERS studies of analytes in the thus generated plasmonic hot-spots. The antennas will also be deposited on lipid membranes where they can be actuated by light-matter interactions.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1032: Nanoagents for Spatio-temporal Control of Molecular and Cellular Reactions
• Applicant Institution: Ludwig-Maximilians-Universität München
• Project Heads: Dr. Amelie Heuer-Jungemann, since 7/2020; Professor Dr. Tim Liedl