Project Details
Projekt
Nucleotide-based activity probes: Dye-labeled ATP-analogues (B08)
Subject Area
Biological and Biomimetic Chemistry
Term
from 2012 to 2019
Project identifier
Deutsche Forschungsgemeinschaft (DFG) - Project number 189682160
The aim of this project is the development of tools for the analysis of protein activity in living cells. For this purpose, chemical synthesis of novel, fluorescently labelled ATP analogues is combined with the implementation of suitable optical live cell imaging techniques. The concept relies on the readout of changes in Förster resonance energy transfer (FRET) with high spatial and temporal resolution. The successful application of this concept has been proven in the first funding period and will be extended further in the future. For this purpose, we will collaborate extensively within the consortium, i.e., concerning ubiquitin activation, kinase and kinesin activities, and protein glycosylations.
DFG Programme
Collaborative Research Centres
Applicant Institution
Universität Konstanz
Project Heads
Professor Dr. Andreas Marx; Professor Dr. Andreas Zumbusch
