Functional analysis of spindle pole body functions during the meiotic cell division in S. cerevisiae
Final Report Abstract
When diploid Saccharomyces cerevisiae cells starve for nutrients, they initiate sporulation to survive adverse environmental conditions. The developmental process of sporulation is coupled to the meiotic cell division, which results in the formation of up to four haploid spores from one diploid mother cell. Reduction in spore numbers is connected to spore abortion; genome inheritance into spores is not random. At the onset of meiosis II, spore formation is initialized at spindle pole bodies, the yeast centrosome equivalents. During meiosis, four spindle pole bodies of three generations (one old, one generated during prophase of meiosis and two generated before the second meiotic division) are present. In undisturbed cells, younger spindle pole bodies are preferred over older ones to be incorporated into a spore, which has consequences on genome inheritance and genetic diversity. We could show that this genome inheritance mechanism and age-based spindle pole body selection depend on the mitotic exit network, a signal transduction pathway important to initiate the final steps of cell division. Our inverstigations revealed that the mitotic exit network acts on distinct targets and at different time-points during spore formation, which demonstrates functional diversification. A major regulatory step, the formation of a meiosisspecific structure at the spindle pole body requires the mitotic exit network, surprisingly the kinases of the network are not localized to the spindle pole body during this regulatory process, but are dispersed in the cytosol. The latter provides evidence for a sporulation-specific activation mechanism of the pathway that is independent from localization at the spindle pole body. Taken together, we demonstrate involvement of the mitotic exit network in age-based spindle pole body inheritance as well as developmental specific remodeling of this signal transduction pathway.
Publications
- (2017) The Mitotic Exit Network Regulates Spindle Pole Body Selection During Sporulation of Saccharomyces cerevisiae. Genetics 206 (2) 919–937
Renicke, Christian; Allmann, Ann-Katrin; Lutz, Anne Pia; Heimerl, Thomas; Taxis, Christof
(See online at https://doi.org/10.1534/genetics.116.194522) - (2013) An optogenetic tool to control protein degradation and cellular function. Chemistry & Biology 20: 619-626
Renicke C, Schuster D, Usherenko S, Essen LO, and Taxis C
- (2016). Biophotography: concepts, applications and perspectives. Appl Microbiol Biotechnol; 100:3415-20
Renicke, C and Taxis, C
(See online at https://doi.org/10.1007/s00253-016-7384-0) - (2016). Controlling protein activity and degradation using blue-light. Methods Mol Biol; 1408:67-78
Lutz AP, Renicke C, and Taxis C
(See online at https://doi.org/10.1007/978-1-4939-3512-3_5) - Development of an optogenetic tool to regulate protein stability in vivo. In: OPTOGENETICS: From Neuronal Function to Mapping & Disease Biology. Appasani K (ed.). Cambridge University Press, Cambridge, pp. 118 - 131
Renicke C, and Taxis C
(See online at https://doi.org/10.1017/9781107281875.011)
