Analyses of eukaryotic ribosome assembly (B01)

• Subject Area: Biochemistry
• Term: from 2011 to 2023
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 185476941

Production of ribosomes is one of the major energy consuming processes in proliferating cells which requires, among others, the correct folding of rRNA and the assembly of ribosomal proteins with rRNA. In the next funding period of the CRC we plan to continue our previous studies on how ribosome biogenesis factors and ribosomal proteins affect the assembly states and stability of ribosomal precursor particles in S. cerevisiae. Apart from this, we want to develop and apply biochemical methods to define the dynamics of the local RNA environment of specific ribosomal proteins and ribosome biogenesis factors in pre-ribosomal particles. We expect that these experiments will enable to correlate specific assembly states with pre-rRNA folding states and will give mechanistic insights into yeast pre-ribosome maturation.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 960: Ribosome Formation: Principles of RNP Biogenesis and Control of their Function
• Applicant Institution: Universität Regensburg
• Project Heads: Privatdozent Dr. Joachim Griesenbeck; Dr. Philipp Milkereit; Professor Dr. Herbert Tschochner