Multiphotonenmikroskop mit Tandemscanner
Final Report Abstract
Since 01.01.2012 the multiphoton microscope (TPLSM) is part of the IPEK optical imaging core facility of atherosclerosis which is located in the Klinikum der LMU Munich. On a collaborational approach, the core facility provides imaging technology and knowledge to internal (ipek) and external (LMU and others) scientists. The TPLSM is mainly used for cardiovascular and immunological experiments that require high resolution imaging deep in intact and viable samples (in situ,) ex vivo and in vivo. Furthermore, due to its flexible layout, the microscope is also being used for imaging of isolated cells in vitro and immunofluorescence histological samples. Finally, the TPLSM is in use to develop novel and improve existing imaging methods for cardiovascular imaging. 1) Visualization of various aspects of the arterial wall (ex vivo, in vivo): Inflammatory cell-vessel wall interactions (neutrophils, monocytes, T-cells). - Thrombocyte-vessel wall (compounds) interactions. - Endothelial cells in the macro- and microcirculation (activation, proliferation, cell-cell junctions, transcytosis). - Vascular smooth muscle cell layers (direction, function). - Visualization of various aspects of atherosclerotic lesions in the aorta and carotid arteries (T-cell interactions, dendritic cells, collagen). 2) Visualization of whole mount tissues and organs (other than arteries): Bone marrow. - Lymph nodes. - Mesenteric lipocytes and inflammation: mesenteric 1st order arteries and inflammatory cells in surrounding lipid tissue. - Tumors. 3) Development of methods: In vivo visualization of cell-vessel wall interactions in the large arteries. - Arterial flow assay in intact and viable (mounted) arteries (ex vivo). - Three-dimensional quantification of vascular smooth muscle orientation. - Tplsm imaging of the lung in vivo.
Publications
- Exchange of extracellular domains of CCR1 and CCR5 reveals confined functions in CCL5-mediated cell recruitment. Thromb Haemost. 2013; 110(4):795-806
Kramp BK, Megens RTA, Sarabi A, Winkler S, Projahn D, Weber C, Koenen RR, von Hundelshausen P
- Neutrophil-Derived Cathelicidin Promotes Adhesion of Classical Monocytes. Circ Res. 2013; 112(5):792-801
Wantha S, Alard JE, Megens RTA, van der Does AM, Döring Y, Drechsler M, Pham CTM, Wang MW, Wang JM, Gallo RL, von Hundelshausen P, Lindbom L, Hackeng T, Weber C, Soehnlein O
- An endothelial microRNA controls flow-dependent susceptibility to atherosclerosis. Nat. Med. 2014. Apr;20(4):368-76
Schober A, Nazari-Jahantigh M, Wei Y, Zhe Z, Gremse F, Grommes J, Megens RTA, Heyll K, Thiemann A, Iruela-Arispe ML, Wang S, Kiessling F, Olson EN, Weber C
(See online at https://doi.org/10.1038/nm.3487) - Endothelial JAM-A guides monocytes into flow-dependent predilection sites of atherosclerosis. Circulation 2014;129(1):66-76
Schmitt MMN, Megens RTA, Zernecke A, Bidzhekov K, van den Akker MN, Rademakers T, van Zandvoort MAMJ, Hackeng TM, Koenen RR, Weber C
(See online at https://doi.org/10.1161/CIRCULATIONAHA.113.004149) - High-Resolution Imaging of Intravascular Atherogenic Inflammation in Live Mice. Circ Res. 2014;114(5):770-9
Chèvre R, González-Granado JM, Megens RTA, Sreeramkumar V, Silvestre-Roig C, Molina-Sanchez P, Weber C, Soehnlein O, Hidalgo A, Andrés V
(See online at https://doi.org/10.1161/CIRCRESAHA.114.302590) - Microvascular dysfunction in the course of metabolic syndrome induced by high-fat diet. Cardiovasc. Diabetol. 2014;3;13:31
Aoqui C, Chmielewski S, Scherer E, Eissler R, Sollinger D, Heid I, Braren R, Schmaderer C, Megens RTA, Weber C, Heemann U, Tschoep M and Baumann M
(See online at https://doi.org/10.1186/1475-2840-13-31) - A method for three-dimensional quantification of vascular smooth muscle orientation: application in viable murine carotid. Biomech Model Mechanobiol. 2015 Jul 15 (Epub ahead of print)
Spronck B, Megens RTA, Reesink S, Delhaas T
(See online at https://doi.org/10.1007/s10237-015-0699-4) - Differential inhibition of human atherosclerotic plaque- induced platelet activation by dimeric GPVI-Fc and anti-GPVI antibodies: functional and imaging studies. J Am Coll Cardiol. 2015; 65(22):2404-15
Jamasbi J, Megens RTA, Bianchini M, Münch G, Ungerer M, Faussner A, Herman S, Walker A, Goyal P, Jung S, Brandl R, Weber C, Lorenz R, Farndale R, Elia N, Siess W
(See online at https://doi.org/10.1016/j.jacc.2015.03.573) - Hyperreactivity of Junctional Adhesion Molecule A–Deficient Platelets Accelerates Atherosclerosis in Hyperlipidemic Mice. Circ Res. 2015;116(4):587-99
Karshovska E, ZhaoZ, Blanchet X, Schmitt MNN, Bidzhekov K, Soehnlein O, von Hundelshausen P, Mattheij NJ, Cosemans JMEM, Megens RTA, Koeppel TA, Schober A, Hackeng TM, Weber C and Koenen RR
(See online at https://doi.org/10.1161/CIRCRESAHA.116.304035)
