Project Details
Projekt
The role of the RIM-binding protein active zone scaffold in regulating neurotransmitter release at diverse central synapses (A05)
Subject Area
Molecular Biology and Physiology of Neurons and Glial Cells
Term
from 2011 to 2023
Project identifier
Deutsche Forschungsgemeinschaft (DFG) - Project number 184695641
How the protein scaffolds that mediates synaptic vesicle exocytosis contributes to the brain wide diversity of synaptic function is largely unknown. We will examine the role of RIM-binding protein in defining the molecular architecture and function in two central murine synapses with known differences in function. We will characterize the RIM-BP2 interactors and their functional implication using biochemistry, in vivo mutagenesis and microscopy. We will examine putative mechanisms for alternative synapse specific scaffold formations such as variations in expression of isoforms of RIM-BP2 and it’s interaction partners, or variation of protein levels. The proposed experiments promise to reveal scaffolding principles that contribute to the functional architecture of the active zone and open up novel understanding of the biology that underlies diversity of synaptic coding.
DFG Programme
Collaborative Research Centres
Applicant Institution
Freie Universität Berlin
Project Heads
Professor Dr. Christian Rosenmund; Professor Dr. Dietmar Schmitz
