Turnover and recycling of the peptidoglycan-teichoic acid complex of Gram-positive cell wall (A15)

• Subject Area: Metabolism, Biochemistry and Genetics of Microorganisms
• Term: from 2011 to 2019
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 32152271

Our goal is to establish the turnover and recycling metabolism of the peptidoglycan-wall teichoic acid (PG-WTA) complex in Bacillus subtilis. We aim to answers the following questions: (i) which fragments are released from the cell wall at the different stages of the cell cycle (elongation, cell division/separation) as well as during spore formation, (ii) which transport proteins and pathways are used to import and reutilize (recycle) these compounds, and (iii) how is cell wall autolysis and recycling regulated, and are particular PG-WTA turnover products used to monitor the state of the cell wall or to time the cell cycle?

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 766: The Bacterial Cell Envelope: Structure, Function and Infection Interface
• Major Instrumentation: Analysator
• Instrumentation Group: 1350 Flüssigkeits-Chromatographen (außer Aminosäureanalysatoren 317), Ionenaustauscher
• Applicant Institution: Eberhard Karls Universität Tübingen
• Project Head: Professor Dr. Christoph Mayer