GRK 1342:  Molecular and Functional Analysis of Lipid-based Signal Transduction Systems

• Subject Area: Plant Sciences
• Term: from 2007 to 2015
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 19719780

• Final Report Year: 2016

Final Report Abstract

The research training group GRK1342 (Molecular and functional analysis of lipid-based signal transduction systems) was established as a research-oriented program that combined advanced research in lipid biology and signal transduction with an interdisciplinary training curriculum for PhD students. It was concerned with the biosynthesis of small molecule lipid signals, the molecular analysis of components of the signal transduction machinery including their arrangement in nanodomains, activation of ion channels and protein kinases/phosphatases, transcriptional regulation of target genes and functional output of lipid-based signals. The impact of structural lipid composition on plant microbe interactions was also studied. Results from these projects helped to explain how, in the context of abiotic and biotic stresses, reactive oxygen species and lipid modifying enzymes rapidly generate biological active lipids that transcriptionally activate detoxification and survival genes to cope better with environmental stress challenges. Beyond their function in stress responses, jasmonate and absicic acid (ABA) lipid signals were found to play an important regulatory role in the hunting cycle and trap performance of the carnivorous Venus flytrap (Dionaea muscipula). Furthermore, first evidence - in plants - that membrane nanodomains represent a platform for functional protein assembly within different signalling complexes was provided. The core ABA-signalosome, consisting of an ABA- receptor/phosphatase complex and anion channel-activating kinases, was reconstituted in vitro, in planta and in the Xenopus oocytes which allowed analyses of the properties of these signaling components and their regulation in membrane lipid nanodomains (rafts). With respect to structural lipids, several projects demonstrated a previously unrecognized impact of cuticle and membrane lipid composition on the phyllosphere microbial community and agrobacteriummediated crown gall disease. Thus, within the research program of the GRK1342, PhD students became familiar with a very broad range of state-of-the-art methods including trancriptomics, proteomics, lipidomics, electrophysiology, optogenetics and high resolution microscopy enabling them to develop a scientific career in academics or industry. Our graduate students further absolved a structured training program comprising lecture series covering a broad range of topics, seminars, conferences and common activities embedded in a vivid plant science community in Würzburg and beyond.

Publications

• (2007) Formation of the indigo precursor indican in genetically engineered tobacco plants and cell cultures. Plant Biotechnol. J. 5: 185-91
  Warzecha, H., Frank, A., Peer, M., Gillam, E., Guengerich, F. and Unger, M.
  
• (2007) Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase. Mol. Cell. Proteomics 6: 1896-906
  Reinders, J., Wagner, K., Zahedi, R.P., Stojanovski, D., Eyrich, B., van der Laan, M., Rehling, P., Sickmann, A., Pfanner, N. and Meisinger, C.
  
• (2007) Proteomic analysis of cathepsin B and L-deficient mouse brain lysosomes. Biochim. Biophys. Acta 1774: 1237-1246
  Stahl, S., Reinders, Y., Asan, E., Mothes, W., Conzelmann, E., Sickmann, A. and Felbor, U.
  
• (2008) Assessment of different expression strategies for the production of a recombinant lipoprotein vaccine in plants. Open Biotechnol. J. 2: 51-55
  Hennig, A., Reinders, Y., Giritch, A., Reinders, J. and Warzecha, H.
  
• (2008) Identification of Arabidopsis thaliana phloem RNAs provides a search criterion for phloem-based transcripts hidden in complex datasets of microarray experiments. Plant J. 55: 746-56
  Deeken, R., Ache, P., Kajahn, I., Klinkenberg, J., Bringmann, G. and Hedrich, R.
  
• (2008). Light regulation and daytime dependency of inducible plant defences in Arabidopsis: phytochrome signalling controls systemic acquired resistance rather than local defence. Plant Physiol. 147: 790-801
  Griebel, T. and Zeier, J.
  
• (2009) A defensin from tomato with dual function in defense and development. Plant Mol. Biol. 71: 131-143
  Stotz, H.U., Wang, Y. and Spence, B.
  
• (2009) Activity of guard cell anion channel SLAC1 is controlled by drought-stress signaling kinase-phosphatase pair. Proc. Natl. Acad. Sci. USA 106: 21425-21430
  Geiger, D., Scherzer, S., Mumm, P., Stange, A., Marten, I., Bauer, H., Ache, P., Matschi, S., Liese, A., Al-Rasheid, K.A.S., Romeis, T. and Hedrich, R.
  
• (2009) Characterization of leaf apoplastic peroxidases and metabolites in Vigna unguiculata in response to toxic manganese supply and silicon. J. Exp. Botany 60: 1663–1678
  Führs, H., Götze, S., Specht, A., Erban, A., Gallien, S., Heintz, D., Van Dorsselaer, A., Kopka, J., Braun, H.-P. and Horst, W.J.
  
• (2009) Novel shuttling domain in a regulator (RSC1A1) of transporter SGLT1 steers cell cycle-dependent nuclear location. Traffic 10: 1599-618
  Filatova, A., Leyerer, M., Gorboulev, V., Chintalapati, C., Reinders, Y., Müller, T.D., Srinivasan, A., Hübner, S. and Koepsell, H.
  
• (2009) Plant defensins: defense, development and application. Plant Signal. Behav. 4: 1010-1013
  Stotz, H.U., Thomson, J. and Wang, Y.
  
• (2009). Methyl salicylate production and jasmonate signaling are not essential for systemic acquired resistance in Arabidopsis. Plant Cell 21: 954-971
  Attaran, E., Zeier, T.E., Griebel, T. and Zeier, J.
  
• (2010) A role for beta-sitosterol to stigmasterol conversion in plantpathogen interactions. Plant J. 63: 254-268
  Griebel, T. and Zeier, J.
  
• (2010) ABA signaling inhibits oxalate-induced production of reactive oxygen species and protects against Sclerotinia sclerotiorum in Arabidopsis thaliana. Eur. J. Plant Pathol. 128: 7-19
  Guo, X. and Stotz, H.U.
  
• (2010) Bioconversion of the antihistaminic drug loratadine by tobacco cell suspension cultures expressing human cytochrome P450 3A4. J. Biosci. Bioeng. 109: 288-290
  Warzecha, H., Ferme, D., Peer, M., Frank, A. and Unger, M.
  
• (2010) Ca2+-dependent activation of guard cell anion channels, triggered by hyperpolarization, is promoted by prolonged depolarization. Plant J. 62: 265-276
  Stange, A., Hedrich, R. and Roelfsema, M.R.G.
  
• (2010) Differential impact of lipoxygenase 2 and jasmonates on natural and stress-induced senescence in Arabidopsis thaliana. Plant Physiol. 152: 1940-1950
  Seltmann, M.A., Stingl, N.E., Lautenschläger, J.K., Krischke, M., Mueller M.J. and Berger, S.
  
• (2010) Guard cell anion channel SLAC1 is regulated by CDPK protein kinases with distinct Ca2+ affinities. Proc. Natl. Acad. Sci. U S A. 107: 8023-8028
  Geiger, D., Scherzer, S., Mumm, P., Marten, I., Ache, P., Matschi, S., Liese, A., Wellmann, C., Al-Rasheid, K.A., Grill, E., Romeis, T. and Hedrich, R.
  
• (2010) Guard Cell Specific Calcium Sensitivity of High Density and Activity SV/TPC1 Channel. Plant Cell Physiol. 51: 1548-1554
  Rienmüller, F., Beyhl, D., Lautner, S., Fromm J., Al-Rasheid, K.A.S., Ache, P., Farmer, E.E., Marten, I. and Hedrich, R.
  
• (2010) Interaction between polygalacturonase-inhibiting protein and jasmonic acid during defense activation in tomato against Botrytis cinerea. Eur. J. Plant Pathol. 128: 423-428
  Akagi, A., Engelberth, J. and Stotz, H.U.
  
• (2010) Jasmonates during senescence: signals or products of metabolism? Plant Signal. Behav. 5: 1493-1496
  Seltmann, M., Hussels and W., Berger S.
  
• (2010) Perception of the Arabidopsis danger signal peptide 1 involves the pattern recognition receptor AtPEPR1 and its close homologue AtPEPR2. J. Biol. Chem. 285: 13471-13479
  Krol, E., Mentzel, T., Chinchilla, D., Boller, T., Felix, G., Kemmerling, B., Postel, S., Arents, M., Jeworutzki, E., Al-Rasheid, K.A., Becker, D. and Hedrich, R.
  
• (2010) Pseudomonas syringae infection triggers de novo sythesis of phytosphingosine from sphinganine in Arabidopsis thaliana. FEBS Lett. 584: 4053-4056
  Peer, M., Stegmann, M., Mueller, M.J., and Waller, F.
  
• (2011) Catch me if you can: mass spectrometry-based phosphoproteomics and quantification strategies. Proteomics 11: 554-570
  Eyrich, B., Sickmann, A., Zahedi, R.P.
  
• (2011) Cytokinins mediate resistance against Pseudomonas syringae in tobacco through increased antimicrobial phytoalexin synthesis independent of salicylic acid signaling. Plant Physiol. 157: 815-30
  Grosskinsky, D.K., Naseem, M.,, Abdelmohsen, U.R., Plickert, N., Engelke, T., Griebel, T., Zeier, J., Novák, O., Strnad, M., Pfeifhofer, H., van der Graaff, E., Simon, U. and Roitsch, T.
  
• (2011) Free sphingobases induce RBOHD-dependent reactive oxygen species production in Arabidopsis leaves. FEBS Lett. 585: 3006-3010
  Peer, M., Bach, M., Müller, M.J. and Waller, F.
  
• (2011) Jasmonate-dependent and COI1-independent responses against Sclerotinia sclerotium in Arabidopsis thaliana: auxin is part of COI1-independent defense signaling. Plant Cell Physiol. 52: 1941-1956
  Stotz, H.U., Jikumaru, Y., Shimada, Y., Sasaki, E., Stingl, N., Mueller, M.J. and Kamiya, Y.
  
• (2011) Regulation of mitochondrial protein import by cytosolic kinases. Cell 144: 227-239
  Schmidt, O., Harbauer, A.B., Rao, S., Eyrich, B., Zahedi, R.P.,Stojanovski, D., Schönfisch, B., Guiard, B., Sickmann, A., Pfanner, N. and Meisinger, C.
  
• (2011) Role of camalexin, indole glucosinolates, and side chain modification of glucosinolate-derived isothiocyanates in defense of Arabidopsis against Sclerotinia sclerotiorum. Plant J. 67: 81-93
  Stotz, H.U., Sawada, Y., Shimada, Y., Hirai, M.Y., Sasaki, E., Krischke, M., Brown, P.D., Saito, K. and Kamiya, Y.
  
• (2011) Stomatal closure by fast abscisic acid signaling is mediated by the guard cell anion channel SLAH3 and the receptor RCAR1. Sci. Signal 4: ra32
  Geiger, D., Maierhofer T., Al-Rasheid, K.A., Scherzer, S., Mumm, P., Liese, A., Ache, P., Wellmann, C., Marten, I., Grill, E., Romeis, T. and Hedrich, R.
  
• (2011): A special pair of phytohormons controls excitability, slow closure, and external stomach formation in the Venus flytrap. Proc. Natl. Acad. Sci. USA 108: 15492-15497
  Escalente-Pérez, M., Krol, E., Stange, A., Geiger, D., Al-Rasheid, K. A., Hause, B., Neher, E. and Hedrich, R.
  
• (2011): High-throughput protoplast transactivation (PTA) system for the analysis of Arabidopsis transcription factor function. Plant J. 68: 560-569
  Wehner, N., Hartmann, L., Ehlert, A., Böttner, S., Oňate-Sánchez, L. and Dröge-Laser, W.
  
• (2012) Lipid profiling of the Arabidopsis hypersensitive response reveals specific lipid peroxidation and fragmentation processes: biogenesis of pimelic and azelaic acid. Plant Physiol. 160: 365-378
  Zoeller, M., Stingl, N., Krischke, M., Fekete, A., Waller, F., Berger, S. and Mueller, M.J.
  (See online at https://doi.org/10.1104/pp.112.202846)
• (2012) Luminal and cytosolic pH feedback on proton pump activity and ATP affinity of V-type ATPase from Arabidopsis. J. Biol. Chem. 287: 8986-8993
  Rienmüller, F., Dreyer, I., Schönknecht, G., Schulz, A., Schumacher, K., Nagy, R., Martinoia, E., Marten, I. and Hedrich, R.
  (See online at https://doi.org/10.1074/jbc.M111.310367)
• (2012) Multiple calciumdependent kinases modulate ABA-activated guard cell anion channels. Mol. Plant. 5: 1409-1412
  Scherzer, S., Maierhofer, T., Al-Rasheid K.A., Geiger, D. and Hedrich, R.
  
• (2012) The Tumor Suppressor DiRas3 Forms a Complex with H-Ras and C-RAF Proteins and Regulates Localization, Dimerization, and Kinase Activity of C-RAF. J. Biol. Chem. 287: 23128- 23140
  Baljuls, A., Beck, M., Oenel, A., Robubi, A., Kroschewski, R., Hekman, M., Rudel, T. and Rapp, U.R.
  (See online at https://doi.org/10.1074/jbc.M112.343780)
• (2012): Methods of staining and visualization of sphingolipid enriched and non-enriched plasma membrane regions of Arabidopsis thaliana with fluorescent dyes and lipid analogues. Plant Methods 8: 28
  Blachutzik, J.O., Demir, F., Kreuzer I., Hedrich, R., Harms, G.S.
  (See online at https://doi.org/10.1186/1746-4811-8-28)
• (2012): Phyllosphere bacterial communities of trichome-bearing and trichomeless Arabidopsis thaliana leaves. Antonie van Leeuwenhoek 101: 551-560
  Reisberg, E.E., Hildebrandt, U., Riederer, M. and Hentschel U.
  (See online at https://doi.org/10.1007/s10482-011-9669-8)
• (2012): Regulation of V-type ATPase by redox modulation. Biochem. J. 448: 243-251
  Seidel, T., Scholl, S., Krebs, M., Rienmüller, F., Marten, I., Hedrich, R., Hanitzsch, M., Janetzki, P., Dietz, K.J. and Schumacher, K.
  (See online at https://doi.org/10.1042/BJ20120976)
• (2013) Analysis of defense signals in Arabidopsis thaliana leaves by ultra-performance liquid chromatography/tandem mass spectrometry: jasmonates, salicyclic acid, abscisic acid. Methods Mol. Biol.1009: 103-113
  Stingl, N., Krischke, M., Fekete, A. and Mueller, M.J.
  
• (2013) Arabidopsis nanodomain-delimited ABA signaling pathway regulates the anion channel SLAH3. Proc. Natl. Acad. Sci. USA 110: 8296-8301
  Demir, F., Horntrich, C., Blachutzik, J.O., Scherzer, S., Reinders, Y., Kierszniowska, S., Schulze, W.X., Harms, G.S., Hedrich, R., Geiger, D. and Kreuzer, I.
  (See online at https://doi.org/10.1073/pnas.1211667110)
• (2013) Conformational changes represent the rate-limiting step in the transport cycle of maize sucrose transporter1. Plant Cell 25: 3010-3021
  Derrer, C., Wittek, A., Bamberg, E., Carpaneto, A., Dreyer, I. and Geiger, D.
  (See online at https://doi.org/10.1105/tpc.113.113621)
• (2013) Lipoxygenase6- dependent oxylipin synthesis in roots is required for abiotic and biotic stress resistance of Arabidopsis. Plant Physiol. 161: 2159-2170
  Grebner, W., Stingl, N.E., Oenel, A., Mueller, M.J. and Berger, S.
  (See online at https://doi.org/10.1104/pp.113.214544)
• (2013) Phenotyping jasmonate regulation of senescence. Methods Mol. Biol. 1011: 3-11
  Seltmann, M.A. and Berger, S.
  
• (2013) Pollen tube growth regulation by free anions depends on the interaction between the anion channel SLAH3 and calcium-dependent protein kinases CPK2 and CPK20. Plant Cell 25: 4525-4543
  Gutermuth, T., Lassig, R., Portes, M.T., Maierhofer, T., Romeis, T., Borst, J.W., Hedrich, R., Feijó, J.A. and Konrad, K.R.
  (See online at https://doi.org/10.1105/tpc.113.118463)
• (2013) Reprogramming of plants during systemic aquired resistance. Front Plant Sci. 15: 252
  Gruner, K., Griebel, T., Návarová H., Attaran, E. and Zeier, J.
  (See online at https://doi.org/10.3389/fpls.2013.00252)
• (2013) Salt stress triggers phosporylation of the Arabidopsis vacuolar K+ channel TPK1 by calcium-dependent protein kinases (CDPKs). Mol. Plant. 6: 1274-1289
  Latz, A., Mehlmer, N., Zapf, S., Mueller, T.D., Wurzinger, B., Pfister, B., Csaszar, E., Hedrich, R., Teige, M. and Becker, D.
  (See online at https://doi.org/10.1093/mp/sss158)
• (2013) TGA transcription factors and jasmonate-independent COI1 signalling regulate specific plant responses to reactive oxylipins. J. Exp. Bot. 64: 963-975
  Stotz, H.U., Mueller, S., Zoeller, M., Mueller, M.J. and Berger, S.
  
• (2013) The Dionea muscipula ammonium channel DmAMT1 provides NH4+ uptake associated with Venus flytrap`s prey digestion. Curr. Biol. 23: 1649-1657
  Scherzer, S., Krol, E., Kreuzer, I., Kruse, J, Karl, F., von Rüden, M., Escalente-Perez, M., Müller, T., Rennenberg, H., Al-Rasheid, K.A., Neher, E. and Hedrich, R.
  (See online at https://doi.org/10.1016/j.cub.2013.07.028)
• (2013): Distinct phyllosphere bacterial communities on Arabidopsis wax mutant leaves. PLoS One 8: e78613
  Reisberg, E.E., Hildebrandt, U., Riederer, M. and Hentschel U.
  (See online at https://doi.org/10.1371/journal.pone.0078613)
• (2014) A single-pore residue renders the Arabidopsis root anion channel SLAH2 highly nitrate selective. Plant Cell 26: 2554-2567
  Maierhofer, T., Lind, C., Hüttl, S., Scherzer, S., Papenfuß, M., Simon, J., Al-Rasheid, K.A., Ache, P., Rennenberg, H., Hedrich, R., Müller, T.D. and Geiger, D.
  (See online at https://doi.org/10.1105/tpc.114.125849)
• (2014) A tandem affinity purification tag of TGA2 for isolation of interacting proteins in Arabidopsis thaliana. Plant Signal Behav. 9:e972794
  Stotz, H.U., Findling, S., Nukarinen, E., Weckwerth, W., Mueller, M.J. and Berger, S.
  (See online at https://doi.org/10.4161/15592316.2014.972794)
• (2014) Actinomycetes from Red Sea sponges: sources for chemical and phylogenetic diversity. Mar. Drugs 12: 2771-2789
  Abdelmohsen, U.R., Yang, C., Horn, H., Hajjar, D., Ravasi, T. and Hentschel, U.
  (See online at https://doi.org/10.3390/md12052771)
• (2014) Computational integration of genomic traits into 16S rDNA microbiota sequencing studies. Gene 549: 186-191
  Keller, A., Horn, H., Förster, F. and Schultz, J.
  (See online at https://doi.org/10.1016/j.gene.2014.07.066)
• (2014) Dereplication strategies for targeted isolation of new antitrypanosomal actinosporins A and B from a marine sponge asociates-Actinokineospora sp. EG49. Mar. Drugs 12: 1220-1244
  Abdelmohsen, U.R., Cheng, C., Viegelmann, C., Zhang, T., Grkovic, T., Ahmed, S., Quinn, R.J., Hentschel, U. and Edrada-Ebel, R.
  (See online at https://doi.org/10.3390/md12031220)
• (2014) Draft genome sequence of the antitrypanosomally active sponge-associated bacterium Actinokineospora sp. Strain EG49. Genome Announcements 2. e00160-14
  Harjes, J., Ryu, T., Abdelmohsen, U.R., Moitinho-Silva, L., Horn, H., Ravasi, T., Hentschel, U.
  (See online at https://dx.doi.org/10.1128/genomeA.00160-14)
• (2014) Kinetics of retrograde signalling initiation in the high light response of Arabidopsis thaliana. Philos. Trans. R. Soc. Lond. B. Biol. Sci. 369: 20130424
  Alsharafa, K., Vogel, M.O., Oelze, M.L., Moore, M., Stingl, N., König, K., Friedman, H., Mueller, M.J., Dietz, K.J.
  (See online at https://doi.org/10.1098/rstb.2013.0424)
• (2014) Manipulation of methyl jasmonate esterase activity renders tomato more susceptible to Sclerotinia sclerotiorum. Func. Plant Biology 41: 133-14
  Findling, S., Fekete, A., Warzecha, H., Krischke, M., Brandt, H., Blume, E., Mueller, M.J. and Berger, S.
  (See online at https://doi.org/10.1071/FP13103)
• (2014) Mechano-stimulation triggers turgor changes associated with trap closure in the Darwin plant Dionaea muscipula. Mol. Plant. 7: 744-746
  Escalente-Pérez, M., Scherzer, S., Al-Rasheid, K.A., Döttinger, C., Neher, E. and Hedrich, R.
  (See online at https://doi.org/10.1093/mp/sst169)
• (2014) Metabolomc tools for secondary metabolite discovery from marine microbial symbionts. Mar. Drugs 12: 3416-3448
  Macintyre, L., Zhang, T., Viegelmann, C., Martinez, I.J., Cheng, C., Dowdells, C., Abdelmohsen, U.R., Gernert, C., Hentschel, U. and Edrada-Ebel, R.
  (See online at https://doi.org/10.3390/md12063416)
• (2014) Pollen tube NAD(P)H oxidases act as a speed control to dampen growth rate oscillations during polarized cell growth. Plant J. 78: 94-106
  Lassig, R., Gutermuth, T., Bey, T.D., Konrad, K.R. and Romeis, T.
  (See online at https://doi.org/10.1111/tpj.12452)
• (2014) Secreted major Venus flytrap chitinase enables digestion of Arthropod prey. Biochim. Biophys. Acta. 1844: 374-383
  Paszota, P., Escalente-Perez, M., Thomsen, L.R., Risør, M.W., Dembski, A., Sanglas, L., Nielsen, T.A., Karring, H., Thøgersen, I.B., Hedrich, R., Enghild, J.J., Kreuzer, I. and Sanggaard, K.W.
  (See online at https://doi.org/10.1016/j.bbapap.2013.11.009)
• (2014) Two fatty acid desaturases, stearoyl-acyl carrier protein Δ9-desaturases6 and fatty acid desaturase3, are involved in drought and hypoxia stress signaling in Arabidopsis crown galls. Plant Physiol. 164: 570-583
  Klingenberg, J., Faist, H., Saupe, S., Lambertz, S., Krischke, M., Stingl, N., Fekete, A., Mueller, M.J., Feussner, I., Hedrich, R. and Deeken, R.
  (See online at https://doi.org/10.1104/pp.113.230326)
• (2014). The low energy signaling network. Front. Plant Sci. 5:1-12
  Tome, F., Nägele, T., Adamo, M., Garg, A., Marco-llorca, C., Nukarinen, E., Pedrotti, L., Peviani, A., Simeunovic, A., Tatkiewicz, A., Tomar, M. and Gamm, M.
  (See online at https://doi.org/10.3389/fpls.2014.00353)
• (2014): Site- and kinase-specific phosphorylation-mediated activation of SLAC1, a guard cell anion channel stimulated by abscisic acid. Sci. Signal 7: ra86
  Maierhofer, T., Diekmann, M., Offenborn, J.N., Lind, C., Bauer, H., Hashimoto, K., S. Al-Rasheid, K.A., Luan, S., Kudla, J., Geiger, D. and Hedrich, R.
  (See online at https://doi.org/10.1126/scisignal.2005703)
• (2015) 2-cysteine peroxiredoxins and thylakoid ascorbate peroxidase create a water-water cycle that ist essential to protect the photosynthetic apparatus under high light stress conditions. Plant Physiol. 167: 1592-1603
  Awad, J., Stotz, H.U., Fekete, A., Krischke, M., Engert, C., Havaux, M., Berger, S. and Mueller, M.J.
  (See online at https://doi.org/10.1104/pp.114.255356)
• (2015) Antichlamydial sterol from the Red Sea sponge Callyspongia aff. implexa. Planta Med. 81: 382-387
  Abdelmohsen, U.R., Cheng, C., Reimer, A., Kozjak-Pavlovic, V., Ibrahim, A.K., Rudel, T., Hentschel, U., Edrada-Ebel, R. and Ahmed S.A.
  (See online at https://doi.org/10.1055/s-0035-1545721)
• (2015) Biodiversity, anti-trypanosomal activity sreening, and metabolomics profiling of actinomycetes isolated from mediterrean sponges. PLoS One: 10(9):e0138528
  Cheng, C., MacIntyre, L., Abdelmohsen, U.R., Horn, H., Polymenakou, P.N., Edrada-Ebel, R. and Hentschel, U.
  (See online at https://doi.org/10.1371/journal.pone.0138528)
• (2015) Calcium sensor kinase activates potassium uptake systems in gland cells of Venus flytraps. Proc. Natl. Acad. Sci. USA 112: 7309-7314
  Scherzer, S., Böhm, J., Krol, E., Shabala, L., Kreuzer, I., Larisch, C., Bemm, F., Al-Rasheid, K.A., Shabala, S., Rennenberg, H., Neher, E. and Hedrich, R.
  (See online at https://doi.org/10.1073/pnas.1507810112)
• (2015) Crosstalk between Two bZIP Signaling Pathways Orchestrates Salt-Induced Metabolic Reprogramming in Arabidopsis Roots. Plant Cell 27: 2244-2260
  Hartmann, L., Pedrotti, L., Weiste, C., Fekete, A., Schierstaedt, J., Göttler, J., Kempa, S., Krischke, M., Dietrich, K., Mueller, M.J., Vicente-Carbajosa, J., Hanson, J. and Dröge-Laser, W.
  (See online at https://doi.org/10.1105/tpc.15.00163)
• (2015) Cytosolic Ca2+-signals enhance the vacuolar ion conductivity of bulging Arabidopsis root hair cells. Mol. Plant. 8: 1665-1674
  Wang, Y., Dindas, J., Rienmüller, F., Krebs, M., Waadt, R., Schumacher, K., Wu W.H., Hedrich, R. and Roelfsema, M.R.
  (See online at https://doi.org/10.1016/j.molp.2015.07.009)
• (2015) Dissection of jasmonate functions in tomato stamen development by transcriptome and metabolome analyses. BMC Biol.13: 28
  Dobritzsch, S., Weyhe, M., Schubert, R., Dindas, J., Hause, G., Kopka, J. and Hause, B.
  (See online at https://doi.org/10.1186/s12915-015-0135-3)
• (2015) Draft genome sequences of three chemically rich actinomycetes isolated from Mediterranean sponges. Marine Genomics 24: 285-287
  Horn, H., Cheng, C., Edrada-Ebel, R.A., Hentschel, U. and Abdelmohsen, U.R.
  (See online at https://doi.org/10.1016/j.margen.2015.10.003)
• (2015) Guard cell SLAC1-type anion channels mediate flagellin-induced stomatal closure. New Phytol. 208: 162-173
  Guzel Deger, A., Scherzer, S., Nuhkat, M., Kedzierska, J., Kollist, H., Brosché, M., Unyayar, S., Boudsocq, M., Hedrich, R. and Roelfsema, M.R.
  (See online at https://doi.org/10.1111/nph.13435)
• (2015) Integration of trap- and root-derived nitrogen nutrition of carnivorous Dionea muscipula. New Phytol. 205: 1320-1329
  Gao, P., Löffler, T.S., Honsel, A., Kruse, J., Krol, E., Scherzer, S., Kreuzer, I., Bemm, F., Buegger, F., Burzlaff, T., Hedrich, R. and Rennenberg, H.
  (See online at https://doi.org/10.1111/nph.13120)
• (2015) K2p channels in plants and animals. Pflugers Arch. 467: 1091-1104
  González, W., Valdebenito, B., Caballero, J., Riadi, G., Riedelsberger, J., Martinez, G., Ramirez, D., Zúñiga, L., Sepúlveda, F.V., Dreyer, I., Janta, M. and Becker, D.
  (See online at https://doi.org/10.1007/s00424-014-1638-4)
• (2015) Mining Genomes of Three Marine Sponge- Associated Actinobacterial Isolates for Secondary metabolism. Genome Announcements 3(5)
  Horn, H., Hentschel, U. and Abdelmohsen, U.R.
  (See online at https://dx.doi.org/10.1128/genomeA.01106-15)
• (2015) Mobility of Group 1b Remorins in Membrane Nanodomains Differs Significantly between Mesophyll Cells and Protoplasts. J Plant Biochem Physiol 3:153
  Blachutzik, J.O., Kreuzer, I., Hedrich, R. and Harms G.S.
  (See online at https://dx.doi.org/10.4172/2329-9029.1000153)
• (2015) SnRK1-triggered switch of bZIP63 dimerization mediates the low-energy response in plants. Elife 4: e05828
  Mair, A., Pedrotti, L., Wurzinger, B., Anrather, D., Simeunovic, A., Weiste, C., Valerio, C., Dietrich, K., Kirchler, T., Nägele, T., Vicente Carbajosa, J., Hanson, J., Baena-González, E., Chaban, C., Weckwerth, W., Dröge-Laser, W. and Teige, M.
  (See online at https://doi.org/10.7554/eLife.05828)
• (2015) Venus flytrap HKT1-type channel provides for prey sodium uptake into carnivorous plant without conflicting with electrical excitability. Mol Plant. 9: 428-436
  Böhm, J., Scherzer, S., Shabala, S., Krol, E., Neher, E., Mueller, T.D. and Hedrich, R.
  (See online at https://doi.org/10.1016/j.molp.2015.09.017)
• Fruit Biology and Disease. In: Dandekar A. (Ed) Fruit Genomics. Verlag John Wiley & Sons (2015)
  Stotz, H.U. and Akagi, A.