EXC 1003:  Cells in Motion - CiM: Imaging to Understand Cellular Behaviour in Organisms

• Subject Area: Basic Research in Biology and Medicine
• Term: from 2012 to 2019
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 194347757

• Final Report Year: 2019

Final Report Abstract

Dynamic cellular behaviour determines development, tissue homeostasis and regeneration. Over the past decade, live imaging strategies have revolutionised our perception of cellular behaviour and emphasised their dynamic nature, a topic to which the ‘Cells in Motion – CiM’ Cluster of Excellence has contributed significantly at national and international levels. Based on its broad interdisciplinary expertise CiM has overcome several existing limitations in optical and medical imaging and has deciphered crucial aspects of cellular behaviour in organisms by analysing systems of increasing complexity. Central to the CiM concept has been the use and development of novel imaging strategies to optimise temporal-spatial resolution and specificity. Our vision remains to promote an interfaculty environment boosting basic biomedical sciences and clinical translation and their interaction using imaging as a common strategy to understand cellular behaviour, regardless of whether this is in cells or tissues, in disease models or in patients. To foster knowledge transfer and scientific creativity we have overcome traditional barriers between faculties by establishing seven new interdisciplinary and two new clinical translational professorships, and a unique Cells in Motion Interfaculty Centre (CiMIC), housed in a new building, the Multiscale Imaging Centre (MIC), supported by WWU funding. Integral to our concept has been a new interfaculty educational structure (Careers in Motion Centre) that promotes gender equality and diversity, and includes a novel Master in ‘Experimental Medicine’ allowing medical students to enter into a structured PhD programme. This will continue to promote high quality clinical research and translation of basic science to the clinic. CiM has successfully and uniquely merged excellence at WWU in cell biology and molecular imaging and integrated interdisciplinary networks between the medical, natural sciences and mathematics faculties as well as the Max Planck Institute for Molecular Biomedicine (MPI), documented in several coordinated national and European programmes. The cluster has been a prime example of a new interactive and long-term sustainable structure linking the participating faculties in research and education. CiM has, thereby, changed the research landscape at WWU and has created a unique academic environment that strongly enhances the national and international visibility of WWU in the field of natural sciences and medicine. It will continue to promote excellence in biomedical research in Germany by the establishment of new Collaborative Research Centres and research networks, supported by CiMIC, and exploiting cutting-edge imaging techniques to address fundamental questions in cell biology and their application to diagnostics and therapy.

Link to the final report

https://doi.org/10.2314/KXP:170112324X

Publications

• Bacteria tracking by in vivo magnetic resonance imaging. BMC Biol. 2013; 11: 63
  Hoerr V, Tuchscherr L, Hüve J, Nippe N, Loser K, Glyvuk N, Tsytsyura Y, Holtkamp M, Sunderkötter C, Karst U, Klingauf J, Peters G, Löffler B, Faber C
  (See online at https://doi.org/10.1186/1741-7007-11-63)
• Blocking endocytosis enhances short-term synaptic depression under conditions of normal availability of vesicles. Neuron. 2013; 80: 343-349
  Hua Y, Woehler A, Kahms M, Haucke V, Neher E, Klingauf J
  (See online at https://doi.org/10.1016/j.neuron.2013.08.010)
• cAMP-induced secretion of endothelial von Willebrand factor is regulated by a phosphorylation/dephosphorylation switch in annexin A2. Blood. 2013; 122: 1042-1051
  Brandherm I, Disse J, Zeuschner D, Gerke V
  (See online at https://doi.org/10.1182/blood-2012-12-475251)
• Endothelial TWIK-related potassium channel-1 (TREK1) regulates immune-cell trafficking into the CNS. Nat Med. 2013; 19: 1161-1165
  Bittner S, Ruck T, Schuhmann MK, Herrmann AM, Moha ou Maati H, Bobak N, Göbel K, Langhauser F, Stegner D, Ehling P, Borsotto M, Pape H-C, Nieswandt B, Kleinschnitz C, Heurteaux C, Galla H-J, Budde T, Wiendl H, Meuth SG
  (See online at https://doi.org/10.1038/nm.3303)
• FIM, a novel FTIR-based imaging method for high throughput locomotion analysis. PLoS One. 2013; 8: e53963
  Risse B, Thomas S, Otto N, Lopmeier T, Valkov D, Jiang X, Klämbt C
  (See online at https://doi.org/10.1371/journal.pone.0053963)
• Alarmin S100A8/S100A9 as a biomarker for molecular imaging of local inflammatory activity. Nat Commun. 2014; 5: 4593
  Vogl T, Eisenblätter M, Völler T, Zenker S, Hermann S, van Lent P, Faust A, Geyer C, Petersen B, Roebrock K, Schäfers M, Bremer C, Roth J
  (See online at https://doi.org/10.1038/ncomms5593)
• Coupling of angiogenesis and osteogenesis by a specific vessel subtype in bone. Nature. 2014; 507: 323-328
  Kusumbe AP, Ramasamy SK, Adams RH
  (See online at https://doi.org/10.1038/nature13145)
• Ena/VASP Proteins Cooperate with the WAVE Complex to Regulate the Actin Cytoskeleton. Dev Cell. 2014; 30: 569-584
  Chen XJ, Squarr AJ, Stephan R, Chen B, Higgins TE, Barry DJ, Martin MC, Rosen MK, Bogdan S, Way M
  (See online at https://doi.org/10.1016/j.devcel.2014.08.001)
• Endothelial Notch activity promotes angiogenesis and osteogenesis in bone. Nature. 2014; 507: 376-380
  Ramasamy SK, Kusumbe AP, Wang L, Adams RH
  (See online at https://doi.org/10.1038/nature13146)
• Fusing VE-Cadherin to alpha-Catenin Impairs Fetal Liver Hematopoiesis and Lymph but Not Blood Vessel Formation. Mol Cell Biol. 2014; 34: 1634-1648
  Dartsch N, Schulte D, Hägerling R, Kiefer F, Vestweber D
  (See online at https://doi.org/10.1128/MCB.01526-13)
• Large Scale RNAi Reveals the Requirement of Nuclear Envelope Breakdown for Nuclear Import of Human Papillomaviruses. PLoS Pathog. 2014; 10: e1004162
  Aydin I, Weber S, Snijder B, Samperio Ventayol P, Kühbacher A, Becker M, Day PM, Schiller JT, Kann M, Pelkmans L, Helenius A, Schelhaas M
  (See online at https://doi.org/10.1371/journal.ppat.1004162)
• Leukocyte extravasation and vascular permeability are each controlled in vivo by different tyrosine residues of VE-cadherin. Nat Immunol. 2014; 15: 223-230
  Wessel F, Winderlich M, Holm M, Frye M, Rivera-Galdos R, Vockel M, Linnepe R, Ipe U, Stadtmann A, Zarbock A, Nottebaum AF, Vestweber D
  (See online at https://doi.org/10.1038/ni.2824)
• Serial F-18-FDG PET/CT distinguishes inflamed from stable plaque phenotypes in shear-stress induced murine atherosclerosis. Atherosclerosis. 2014; 234: 276-282
  Wenning C, Kloth C, Kuhlmann MT, Jacobs AH, Schober O, Hermann S, Schäfers MA
  (See online at https://doi.org/10.1016/j.atherosclerosis.2014.03.008)
• The Drosophila FHOD1-like formin Knittrig acts through Rok to promote stress fiber formation and directed macrophage migration during the cellular immune response. Development. 2014; 141: 1366-1380
  Lammel U, Bechtold M, Risse B, Berh D, Fleige A, Bunse I, Jiang X, Klämbt C, Bogdan S
  (See online at https://doi.org/10.1242/dev.101352)
• Total variation regularization in measurement and image space for PET reconstruction. Inverse Probl. 2014; 30: 105003
  Burger M, Müller J, Papoutsellis E, Schönlieb CB
  (See online at https://doi.org/10.1088/0266-5611/30/10/105003)
• Ultraviolet B light attenuates the systemic immune response in central nervous system autoimmunity. Ann Neurol. 2014; 75: 739-758
  Breuer J, Schwab N, Schneider-Hohendorf T, Marziniak M, Mohan H, Bhatia U, Gross CC, Clausen BE, Weishaupt C, Luger TA, Meuth SG, Loser K, Wiendl H
  (See online at https://doi.org/10.1002/ana.24165)
• Viral suppressors of the RIG-I-mediated interferon response are pre-packaged in influenza virions. Nat Commun. 2014; 5: 5645
  Liedmann S, Hrincius ER, Guy C, Anhlan D, Dierkes R, Carter R, Wu G, Staeheli P, Green DR, Wolff T, McCullers JA, Ludwig S, Ehrhardt C
  (See online at https://doi.org/10.1038/ncomms6645)
• VLA-4 blockade promotes differential routes into human CNS involving PSGL-1 rolling of T cells and MCAM- adhesion of TH17 cells. J Exp Med. 2014; 211: 1833-1846
  Schneider-Hohendorf T, Rossaint J, Mohan H, Böning D, Breuer J, Kuhlmann T, Gross CC, Flanagan K, Sorokin L, Vestweber D, Zarbock A, Schwab N, Wiendl H
  (See online at https://doi.org/10.1084/jem.20140540)
• A Bio-Inspired, Catalytic E --> Z Isomerization of Activated Olefins. J Am Chem Soc. 2015; 137: 11254-11257
  Metternich JB, Gilmour R
  (See online at https://doi.org/10.1021/jacs.5b07136)
• A Dynamic Unfolded Protein Response Contributes to the Control of Cortical Neurogenesis. Developmental Cell. 2015; 35: 553-567
  Laguesse S, Creppe C, Nedialkova DD, Prévot PP, Borgs L, Huysseune S, Franco B, Duysens G, Krusy N, Lee G, Thelen N, Thiry M, Close P, Chariot A, Malgrange B, Leidel SA, Godin J D, Nguyen L
  (See online at https://doi.org/10.1016/j.devcel.2015.11.005)
• Blocking neutrophil diapedesis prevents hemorrhage during thrombocytopenia. J Exp Med. 2015; 212: 1255-1266
  Hillgruber C, Pöppelmann B, Weishaupt C, Steingräber AK, Wessel F, Berdel WE, Gessner JE, Ho-Tin-Noe B, Vestweber D, Goerge T
  (See online at https://doi.org/10.1084/jem.20142076)
• CD8+ T cell-mediated neuronal dysfunction and degeneration in limbic encephalitis. Front Neurol. 2015; 6: 163
  Ehling P, Melzer N, Budde T, Meuth SG
  (See online at https://doi.org/10.3389/fneur.2015.00163)
• Differential phospholipase C-dependent modulation of TASK and TREK two-pore domain K+ channels in rat thalamocortical relay neurons. J Physiol. 2015; 593: 127-144
  Bista P, Pawlowski M, Cerina M, Ehling P, Leist M, Meuth P, Aissaoui A, Borsotto M, Heurteaux C, Decher N, Pape H-C, Oliver D, Meuth SG, Budde T
  (See online at https://doi.org/10.1113/jphysiol.2014.276527)
• Focal MMP-2 and MMP-9 Activity at the Blood-Brain Barrier Promotes Chemokine-Induced Leukocyte Migration. Cell Rep. 2015; 10: 1040-1054
  Song J, Wu C, Korpos E, Zhang X, Agrawal SM, Wang Y, Faber C, Schäfers M, Körner H, Opdenakker G, Hallmann R, Sorokin L
  (See online at https://doi.org/10.1016/j.celrep.2015.01.037)
• Influence of cell shape, inhomogeneities and diffusion barriers in cell polarization models. Phys Biol. 2015; 12: 066014
  Giese W, Eigel M, Westerheide S, Engwer C, Klipp E
  (See online at https://doi.org/10.1088/1478-3975/12/6/066014)
• Interfering with VE-PTP stabilizes endothelial junctions in vivo via Tie-2 in the absence of VE-cadherin. J Exp Med. 2015; 212: 2267-2287
  Frye M, Dierkes M, Küppers V, Vockel M, Tomm J, Zeuschner D, Rossaint J, Zarbock A, Koh GY, Peters K, Nottebaum AF, Vestweber D
  (See online at https://doi.org/10.1084/jem.20150718)
• JAM-A regulates cortical dynein localization through Cdc42 to control planar spindle orientation during mitosis. Nat Commun. 2015; 6: 8128
  Tuncay H, Brinkmann BF, Steinbacher T, Schurmann A, Gerke V, Iden S, Ebnet K
  (See online at https://doi.org/10.1038/ncomms9128)
• Multimodal imaging reveals temporal and spatial microglia and matrix metalloproteinase activity after experimental stroke. J Cereb Blood Flow Metab.2015; 35: 1711-1721
  Zinnhardt B, Viel T, Wachsmuth L, Vrachimis A, Wagner S, Breyholz H-J, Faust A, Hermann S, Kopka K, Faber C, Dolle F, Pappata S, Planas AM, Tavitian B, Schäfers M, Sorokin LM, Kuhlmann MT, Jacobs AH
  (See online at https://doi.org/10.1038/jcbfm.2015.149)
• Optimization of Codon Translation Rates via tRNA Modifications Maintains Proteome Integrity. Cell. 2015; 161: 1606-1618
  Nedialkova DD, Leidel SA
  (See online at https://doi.org/10.1016/j.cell.2015.05.022)
• Puzzle Approach to Pose Tracking of a Rigid Object in a Multi Camera System. Lect Notes Comput Sc. 2015; 9256: 337-349
  Schmid S, Jiang X, Schäfers K
  (See online at https://doi.org/10.1007/978-3-319-23192-1_28)
• The motorized RhoGAP myosin IXb (Myo9b) in leukocytes regulates experimental autoimmune encephalomyelitis induction and recovery. J Neuroimmunol. 2015; 282: 25-32
  Liu Z, Xu Y, Zhang X, Song J, Sorokin L, Bähler M
  (See online at https://doi.org/10.1016/j.jneuroim.2015.03.014)
• The Triple-Repeat Protein Anakonda Controls Epithelial Tricellular Junction Formation in Drosophila. Dev Cell. 2015; 33: 535-548
  Byri S, Misra T, Syed ZA, Bätz T, Shah J, Boril L, Glashauser J, Aegerter-Wilmsen T, Matzat T, Moussian B, Uv A, Luschnig S
  (See online at https://doi.org/10.1016/j.devcel.2015.03.023)
• A novel family of fluorescent hypoxia sensors reveal strong heterogeneity in tumor hypoxia at the cellular level. Embo J. 2016; 35: 102-113
  Erapaneedi R, Belousov VV, Schäfers M, Kiefer F
  (See online at https://doi.org/10.15252/embj.201592775)
• Age-dependent modulation of vascular niches for haematopoietic stem cells. Nature. 2016; 532: 380-384
  Kusumbe AP, Ramasamy SK, Itkin T, Mäe MA, Langen UH, Betsholtz C, Lapidot T, Adams RH
  (See online at https://doi.org/10.1038/nature17638)
• Cannabinoid Receptor 2 Modulates Susceptibility to Experimental Cerebral Malaria through a CCL17-dependent Mechanism. J Biol Chem. 2016; 291: 19517-19531
  Alferink J, Specht S, Arends H, Schumak B, Schmidt K, Ruland C, Lundt R, Kemter A, Dlugos A, Kuepper JM, Poppensieker K, Findeiss M, Albayram O, Otte D-M, Marazzi J, Gertsch J, Forster I, Maier W, Scheu S, Hoerauf A, Zimmer A
  (See online at https://doi.org/10.1074/jbc.M116.746594)
• Combinatorial effects of doxorubicin and retargeted tissue factor by intratumoral entrapment of doxorubicin and proapoptotic increase of tumor vascular infarction. Oncotarget. 2016; 7: 82458-82472
  Stucke-Ring J, Ronnacker J, Brand C, Höltke C, Schliemann C, Kessler T, Schmidt LH, Harrach S, Mantke V, Hintelmann H, Hartmann W, Wardelmann E, Lenz G, Wünsch B, Müller-Tidow C, Mesters RM, Schwöppe C, Berdel WE
  (See online at https://doi.org/10.18632/oncotarget.12559)
• Directed transport of neutrophil-derived extracellular vesicles enables platelet-mediated innate immune response. Nat Commun. 2016; 7: 13464
  Rossaint J, Kühne K, Skupski J, Van Aken H, Looney MR, Hidalgo A, Zarbock A
  (See online at https://doi.org/10.1038/ncomms13464)
• Equilibrium physics breakdown reveals the active nature of red blood cell flickering. Nat Phys. 2016; 12: 513-519
  Turlier H, Fedosov DA, Audoly B, Auth T, Gov NS, Sykes C, Joanny J-F, Gompper G, Betz T
  (See online at https://doi.org/10.1038/NPHYS3621)
• FGF23 signaling impairs neutrophil recruitment and host defense during CKD. J Clin Invest. 2016; 126: 962-974
  Rossaint J, Oehmichen J, Van Aken H, Reuter S, Pavenstädt H, Meersch M, Unruh M, Zarbock A
  (See online at https://doi.org/10.1172/JCI83470)
• Fluorinated matrix metalloproteinases inhibitors-Phosphonate based potential probes for positron emission tomography. Bioorg Med Chem. 2016; 24: 902-909
  Beutel B, Daniliuc CG, Riemann B, Schäfers M, Haufe G
  (See online at https://doi.org/10.1016/j.bmc.2016.01.017)
• Gemcitabine Mechanism of Action Confounds Early Assessment of Treatment Response by 3'-Deoxy-3'-[18F]Fluorothymidine in Preclinical Models of Lung Cancer. Cancer Res. 2016; 76: 7096-7105
  Schelhaas S, Held A, Wachsmuth L, Hermann S, Honess DJ, Heinzmann K, Smith D-M, Griffiths JR, Faber C, Jacobs AH
  (See online at https://doi.org/10.1158/0008-5472.CAN-16-1479)
• Imaging by Elemental and Molecular Mass Spectrometry Reveals the Uptake of an Arsenolipid in the Brain of Drosophila melanogaster. Anal Chem. 2016; 88: 5258-5263
  Niehoff A-C, Schulz J, Soltwisch J, Meyer S, Kettling H, Sperling M, Jeibmann A, Dreisewerd K, Francesconi KA, Schwerdtle T, Karst U
  (See online at https://doi.org/10.1021/acs.analchem.6b00333)
• Imaging matrix metalloproteinase activity in multiple sclerosis as a specific marker of leukocyte penetration of the blood-brain barrier. Sci Transl Med. 2016; 8: 364ra152
  Gerwien H, Hermann S, Zhang X, Korpos E, Song J, Kopka K, Faust A, Wenning C, Gross CC, Honold L, Melzer N, Opdenakker G, Wiendl H, Schäfers M, Sorokin L
  (See online at https://doi.org/10.1126/scitranslmed.aaf8020)
• Impaired NK-mediated regulation of T-cell activity in multiple sclerosis is reconstituted by IL-2 receptor modulation. Proc Natl Acad Sci USA. 2016; 113: E2973-82
  Gross CC, Schulte-Mecklenbeck A, Runzi A, Kuhlmann T, Posevitz-Fejfar A, Schwab N, Schneider-Hohendorf T, Herich S, Held K, Konjevic M, Hartwig M, Dornmair K, Hohlfeld R, Ziemssen T, Klotz L, Meuth SG, Wiendl H
  (See online at https://doi.org/10.1073/pnas.1524924113)
• Labeling and Selective Inactivation of Gram-Positive Bacteria Employing Bimodal Photoprobes with Dual Readouts. Chemistry. 2016; 22: 5243-5252
  Galstyan A, Block D, Niemann S, Grüner MC, Abbruzzetti S, Oneto M, Daniliuc CG, Hermann S, Viappiani C, Schäfers M, Löffler B, Strassert CA, Faust A
  (See online at https://doi.org/10.1002/chem.201504935)
• Melanocortin-1 receptor activation is neuroprotective in mouse models of neuroinflammatory disease. Sci Transl Med. 2016; 8: 362ra146
  Mykicki N, Herrmann AM, Schwab N, Deenen R, Sparwasser T, Limmer A, Wachsmuth L, Klotz L, Köhrer K, Faber C, Wiendl H, Luger TA, Meuth SG, Loser K
  (See online at https://doi.org/10.1126/scitranslmed.aaf8732)
• Modifying the 5'-Cap for Click Reactions of Eukaryotic mRNA and To Tune Translation Efficiency in Living Cells. Angew Chem Int Ed Engl. 2016; 55: 10899-10903
  Holstein JM, Anhäuser L, Rentmeister A
  (See online at https://doi.org/10.1002/anie.201604107)
• Protection from septic peritonitis by rapid neutrophil recruitment through omental high endothelial venules. Nat Commun. 2016; 7: 10828
  Buscher K, Wang H, Zhang X, Striewski P, Wirth B, Saggu G, Lütke-Enking S, Mayadas TN, Ley K, Sorokin L, Song J
  (See online at https://doi.org/10.1038/ncomms10828)
• Repulsive cues combined with physical barriers and cell-cell adhesion determine progenitor cell positioning during organogenesis. Nat Commun. 2016; 7: 11288
  Paksa A, Bandemer J, Hoeckendorf B, Razin N, Tarbashevich K, Minina S, Meyen D, Biundo A, Leidel SA, Peyrieras N, Gov NS, Keller PJ, Raz E
  (See online at https://doi.org/10.1038/ncomms11288)
• Repulsive Epithelial Cues Direct Glial Migration along the Nerve. Dev Cell. 2016; 39: 696-707
  Sasse S, Klämbt C
  (See online at https://doi.org/10.1016/j.devcel.2016.11.016)
• Staccato/Unc-13-4 controls secretory lysosome-mediated lumen fusion during epithelial tube anastomosis. Nat Cell Biol. 2016; 18: 727-739
  Caviglia S, Brankatschk M, Fischer EJ, Eaton S, Luschnig S
  (See online at https://doi.org/10.1038/ncb3374)
• A central region in the minor capsid protein of papillomaviruses facilitates viral genome tethering and membrane penetration for mitotic nuclear entry. PLoS Pathog. 2017; 13: e1006308
  Aydin I, Villalonga-Planells R, Greune L, Bronnimann MP, Calton CM, Becker M, Lai K-Y, Campos SK, Schmidt MA, Schelhaas M
  (See online at https://doi.org/10.1371/journal.ppat.1006308)
• A Novel Mouse Model of Staphylococcus aureus Vascular Graft Infection: Noninvasive Imaging of Biofilm Development in Vivo. Am J Pathol. 2017; 187: 268-279
  Van de Vyver H, Bovenkamp PR, Hoerr V, Schwegmann K, Tuchscherr L, Niemann S, Kursawe L, Grosse C, Moter A, Hansen U, Neugebauer U, Kuhlmann MT, Peters G, Hermann S, Löffler B
  (See online at https://doi.org/10.1016/j.ajpath.2016.10.005)
• An Evolutionarily Conserved Role for Polydom/Svep1 During Lymphatic Vessel Formation. Circ Res. 2017; 120: 1263-1275
  Karpanen T, Padberg Y, van de Pavert SA, Dierkes C, Morooka N, Peterson-Maduro J, van de Hoek G, Adrian M, Mochizuki N, Sekiguchi K, Kiefer F, Schulte D, Schulte-Merker S
  (See online at https://doi.org/10.1161/CIRCRESAHA.116.308813)
• An FIM-Based Long-Term In-Vial Monitoring System for Drosophila Larvae. IEEE Trans Biomed Eng. 2017; 64: 1862-1874
  Berh D, Risse B, Michels T, Otto N, Jiang X, Klämbt C
  (See online at https://doi.org/10.1109/TBME.2016.2628203)
• Bleb Expansion in Migrating Cells Depends on Supply of Membrane from Cell Surface Invaginations. Dev Cell. 2017; 43: 577-587.e5
  Goudarzi M, Tarbashevich K, Mildner K, Begemann I, Garcia J, Paksa A, Reichman-Fried M, Mahabaleshwar H, Blaser H, Hartwig J, Zeuschner D, Galic M, Bagnat M, Betz T, Raz E
  (See online at https://doi.org/10.1016/j.devcel.2017.10.030)
• Cell-matrix signals specify bone endothelial cells during developmental osteogenesis. Nat Cell Biol. 2017; 19: 189-201
  Langen UH, Pitulescu ME, Kim JM, Enriquez-Gasca R, Sivaraj KK, Kusumbe AP, Singh A, Di Russo J, Bixel MG, Zhou B, Sorokin L, Vaquerizas JM, Adams RH
  (See online at https://doi.org/10.1038/ncb3476)
• Changing POU dimerization preferences converts Oct6 into a pluripotency inducer. EMBO Rep. 2017; 18: 319-333
  Jerabek S, Ng CK, Wu G, Arauzo-Bravo MJ, Kim K-P, Esch D, Malik V, Chen Y, Velychko S, MacCarthy CM, Yang X, Cojocaru V, Schöler HR, Jauch R
  (See online at https://doi.org/10.15252/embr.201642958)
• Combined PET Imaging of the Inflammatory Tumor Microenvironment Identifies Margins of Unique Radiotracer Uptake. Cancer Res. 2017; 77: 1831-1841
  Zinnhardt B, Pigeon H, Theze B, Viel T, Wachsmuth L, Fricke IB, Schelhaas S, Honold L, Schwegmann K, Wagner S, Faust A, Faber C, Kuhlmann MT, Hermann S, Schäfers M, Winkeler A, Jacobs AH
  (See online at https://doi.org/10.1158/0008-5472.CAN-16-2628)
• Dll4 and Notch signalling couples sprouting angiogenesis and artery formation. Nat Cell Biol. 2017; 19: 915-927
  Pitulescu ME, Schmidt I, Giaimo BD, Antoine T, Berkenfeld F, Ferrante F, Park H, Ehling M, Biljes D, Rocha SF, Langen UH, Stehling M, Nagasawa T, Ferrara N, Borggrefe T, Adams RH
  (See online at https://doi.org/10.1038/ncb3555)
• Endoglin controls blood vessel diameter through endothelial cell shape changes in response to haemodynamic cues. Nat Cell Biol. 2017; 19: 653-665
  Sugden WW, Meissner R, Aegerter-Wilmsen T, Tsaryk R, Leonard EV, Bussmann J, Hamm MJ, Herzog W, Jin Y, Jakobsson L, Denz C, Siekmann AF
  (See online at https://doi.org/10.1038/ncb3528)
• Endothelial basement membrane laminin 511 is essential for shear stress response. EMBO J. 2017; 36: 183-201
  Di Russo J, Luik A-L, Yousif L, Budny S, Oberleithner H, Hofschröer V, Klingauf J, van Bavel E, Bakker E NTP, Hellstrand P, Bhattachariya A, Albinsson S, Pincet F, Hallmann R, Sorokin L
  (See online at https://doi.org/10.15252/embj.201694756)
• Endothelial Notch signalling limits angiogenesis via control of artery formation. Nat Cell Biol. 2017; 19: 928-940
  Hasan SS, Tsaryk R, Lange M, Wisniewski L, Moore JC, Lawson ND, Wojciechowska K, Schnittler H, Siekmann AF
  (See online at https://doi.org/10.1038/ncb3574)
• FIM2c : A Multicolour, Multipurpose Imaging System to Manipulate and Analyse Animal Behaviour. IEEE Trans Biomed Eng. 2017; 64: 610-620
  Risse B, Otto N, Berh D, Jiang X, Kiel M, Klämbt C
  (See online at https://doi.org/10.1109/TBME.2016.2570598)
• FIMTrack: An open source tracking and locomotion analysis software for small animals. PLoS Comput Biol. 2017; 13: e1005530
  Risse B, Berh D, Otto N, Klämbt C, Jiang X
  (See online at https://doi.org/10.1371/journal.pcbi.1005530)
• Generic wound signals initiate regeneration in missing-tissue contexts. Nat Commun. 2017; 8: 2282
  Owlarn S, Klenner F, Schmidt D, Rabert F, Tomasso A, Reuter H, Mulaw MA, Moritz S, Gentile L, Weidinger G, Bartscherer K
  (See online at https://doi.org/10.1038/s41467-017-02338-x)
• Mutations in KEOPS-complex genes cause nephrotic syndrome with primary microcephaly. Nat Genet. 2017; 49: 1529-1538
  Braun DA, ..., Hildebrandt F
  (See online at https://doi.org/10.1038/ng.3933)
• PAR-1 promotes microtubule breakdown during dendrite pruning in Drosophila. EMBO J. 2017; 36: 1981-1991
  Herzmann S, Krumkamp R, Rode S, Kintrup C, Rumpf S
  (See online at https://doi.org/10.15252/embj.201695890)
• Polarized actin and VE-cadherin dynamics regulate junctional remodelling and cell migration during sprouting angiogenesis. Nat Commun. 2017; 8 (1): 2210
  Cao J, Ehling M, März S, Seebach J, Tarbashevich K, Sixta T, Pitulescu ME, Werner A-C, Flach B, Montanez E, Raz E, Adams RH, Schnittler H
  (See online at https://doi.org/10.1038/s41467-017-02373-8)
• Radiolabeled Selective Matrix Metalloproteinase 13 (MMP-13) Inhibitors: (Radio)Syntheses and in Vitro and First in Vivo Evaluation. J Med Chem. 2017; 60: 307-321
  Hugenberg V, Wagner S, Kopka K, Schäfers M, Schuit RC, Windhorst AD, Hermann S
  (See online at https://doi.org/10.1021/acs.jmedchem.6b01284)
• Reversible Stabilization of Vesicles: Redox-Responsive Polymer Nanocontainers for Intracellular Delivery. Angew Chem Int Ed Engl. 2017; 56: 9603-9607
  de Vries WC, Grill D, Tesch M, Ricker A, Nüsse H, Klingauf J, Studer A, Gerke V, Ravoo BJ
  (See online at https://doi.org/10.1002/anie.201702620)
• S100-alarmin-induced innate immune programming protects newborn infants from sepsis. Nat Immunol. 2017; 18: 622-632
  Ulas T, Pirr S, Fehlhaber B, Bickes MS, Loof TG, Vogl T, Mellinger L, Heinemann AS, Burgmann J, Schöning J, Schreek S, Pfeifer S, Reuner F, Völlger L, Stanulla M, von Köckritz-Blickwede M, Glander S, Barczyk-Kahlert K, von Kaisenberg CS, Friesenhagen J, Fischer-Riepe L, Zenker S, Schultze JL, Roth J, Viemann D
  (See online at https://doi.org/10.1038/ni.3745)
• VGLUT1 functions as a glutamate/proton exchanger with chloride channel activity in hippocampal glutamatergic synapses. Nat Commun. 2017; 8: 2279
  Martineau M, Guzman RE, Fahlke C, Klingauf J
  (See online at https://doi.org/10.1038/s41467-017-02367-6)
• VIPAR, a quantitative approach to 3D histopathology applied to lymphatic malformations. JCI Insight. 2017; 2
  Hägerling R, Drees D, Scherzinger A, Dierkes C, Martin-Almedina S, Butz S, Gordon K, Schäfers M, Hinrichs K, Ostergaard P, Vestweber D, Goerge T, Mansour S, Jiang X, Mortimer PS, Kiefer F
  (See online at https://doi.org/10.1172/jci.insight.93424)
• Addressable Cholesterol Analogs for Live Imaging of Cellular Membranes. Cell Chem Biol. 2018; 25: 952-961.e12
  Rakers L, Grill D, Matos ALL, Wulff S, Wang D, Börgel J, Körsgen M, Arlinghaus HF, Galla H-J, Gerke V, Glorius F
  (See online at https://doi.org/10.1016/j.chembiol.2018.04.015)
• Autoinhibitory regulation of S100A8/S100A9 alarmin activity locally restricts sterile inflammation. J Clin Invest. 2018; 128: 1852-1866
  Vogl T, Stratis A, Wixler V, Völler T, Thurainayagam S, Jorch SK, Zenker S, Dreiling A, Chakraborty D, Fröhling M, Paruzel P, Wehmeyer C, Hermann S, Papantonopoulou O, Geyer C, Loser K, Schäfers M, Ludwig S, Stoll M, Leanderson T, Schultze JL, König S, Pap T, Roth J
  (See online at https://doi.org/10.1172/JCI89867)
• CD99L2 deficiency inhibits leukocyte entry into the central nervous system and ameliorates neuroinflammation. J Leukoc Biol. 2018; 104: 787-797
  Samus M, Seelige R, Schäfer K, Sorokin L, Vestweber D
  (See online at https://doi.org/10.1002/JLB.1A0617-228R)
• Codon-specific translation reprogramming promotes resistance to targeted therapy. Nature. 2018; 558: 605-609
  Rapino F, Delaunay S, Rambow F, Zhou Z, Tharun L, De Tullio P, Sin O, Shostak K, Schmitz S, Piepers J, Ghesquiere B, Karim L, Charloteaux B, Jamart D, Florin A, Lambert C, Rorive A, Jerusalem G, Leucci E, Dewaele M, Vooijs M, Leidel SA, Georges M, Voz M, Peers B, Büttner R, Marine J-C, Chariot A, Close P
  (See online at https://doi.org/10.1038/s41586-018-0243-7)
• Collective Cell Migration in Embryogenesis Follows the Laws of Wetting. Biophys J. 2018; 114: 213-222
  Wallmeyer B, Trinschek S, Yigit S, Thiele U, Betz T
  (See online at https://doi.org/10.1016/j.bpj.2017.11.011)
• Distinct roles of VE‐cadherin for development and maintenance of specific lymph vessel beds. EMBO J. 2018: e98271
  Hägerling R, Hoppe E, Dierkes C, Stehling M, Makinen T, Butz S, Vestweber D, Kiefer F
  (See online at https://doi.org/10.15252/embj.201798271)
• Enzymatic or In Vivo Installation of Propargyl Groups in Combination with Click Chemistry for the Enrichment and Detection of Methyltransferase Target Sites in RNA. Angew Chem Int Ed Engl. 2018; 57: 6342-6346
  Hartstock K, Nilges BS, Ovcharenko A, Cornelissen NV, Püllen N, Lawrence-Dörner A-M, Leidel SA, Rentmeister A
  (See online at https://doi.org/10.1002/anie.201800188)
• Harnessing the Maltodextrin Transport Mechanism for Targeted Bacterial Imaging: Structural Requirements for Improved in vivo Stability in Tracer Design. Chem Med Chem. 2018; 13: 241-250
  Axer A, Hermann S, Kehr G, Clases D, Karst U, Fischer-Riepe L, Roth J, Fobker M, Schäfers M, Gilmour R, Faust A
  (See online at https://doi.org/10.1002/cmdc.201700543)
• Human T cells in silico: Modelling dynamic intracellular calcium and its influence on cellular electrophysiology. J Immunol Methods. 2018; 461: 78-84
  Eichinger P, Herrmann AM, Ruck T, Herty M, Gola L, Kovac S, Budde T, Meuth SG, Hundehege P
  (See online at https://doi.org/10.1016/j.jim.2018.06.020)
• Imaging, myeloid precursor immortalization, and genome editing for defining mechanisms of leukocyte recruitment in vivo. Theranostics. 2018; 8: 2407-2423
  Gran S, Honold L, Fehler O, Zenker S, Eligehausen S, Kuhlmann MT, Geven E, den Bosch M van, van Lent P, Spiekermann C, Hermann S, Vogl T, Schäfers M, Roth J
  (See online at https://doi.org/10.7150/thno.23632)
• Lateral plasma membrane compartmentalization links protein function and turnover. Embo J. 2018; 37: e99473
  Busto JV, Elting A, Haase D, Spira F, Kuhlman J, Schäfer-Herte M, Wedlich-Söldner R
  (See online at https://doi.org/10.15252/embj.201899473)
• Molecular imaging of MMP activity discriminates unstable from stable plaque phenotypes in shear-stress induced murine atherosclerosis. PLoS One. 2018; 13: e0204305
  Seifert R, Kuhlmann MT, Eligehausen S, Kiefer F, Hermann S, Schäfers M
  (See online at https://doi.org/10.1371/journal.pone.0204305)
• New in Vivo Compatible Matrix Metalloproteinase (MMP)-2 and MMP-9 Inhibitors. Bioconjug Chem. 2018; 29: 3715-3725
  Beutel B, Song J, Konken CP, Korpos E, Schinor B, Gerwien H, Vidyadharan R, Burmeister M, Li L, Haufe G, Sorokin L
  (See online at https://doi.org/10.1021/acs.bioconjchem.8b00618)
• Optimal Micropatterns in 2D Transport Networks and Their Relation to Image Inpainting. Arch Rational Mech Anal. 2018; 228: 279-308
  Brancolini A, Rossmanith C, Wirth B
  (See online at https://doi.org/10.1007/s00205-017-1192-2)
• Polarized microtubule dynamics directs cell mechanics and coordinates forces during epithelial morphogenesis. Nat Cell Biol. 2018; 20: 1126-1133
  Singh A, Saha T, Begemann I, Ricker A, Nüsse H, Thorn-Seshold O, Klingauf J, Galic M, Matis M
  (See online at https://doi.org/10.1038/s41556-018-0193-1)
• The sulfite oxidase Shopper controls neuronal activity by regulating glutamate homeostasis in Drosophila ensheathing glia. Nat Commun. 2018; 9: 3514
  Otto N, Marelja Z, Schoofs A, Kranenburg H, Bittern J, Yildirim K, Berh D, Bethke M, Thomas S, Rode S, Risse B, Jiang X, Pankratz M, Leimkühler S, Klämbt C
  (See online at https://doi.org/10.1038/s41467-018-05645-z)
• An RJMCMC-Based Method for Tracking and Resolving Collisions of Drosophila Larvae. IEEE/ACM Trans Comput Biol Bioinform. 2019; 16: 465-474
  Michels T, Berh D, Jiang X
  (See online at https://doi.org/10.1109/TCBB.2017.2779141)
• Development of Novel Quinoxaline-Based kappa-Opioid Receptor Agonists for the Treatment of Neuroinflammation. J Med Chem. 2019; 62: 893-907
  Tangherlini G, Kalinin DV, Schepmann D, Che T, Mykicki N, Ständer S, Loser K, Wünsch B
  (See online at https://doi.org/10.1021/acs.jmedchem.8b01609)
• EZH2 Inhibition in Ewing Sarcoma Upregulates GD2 Expression for Targeting with Gene-Modified T Cells. Mol Ther. 2019; 27: 933-946
  Kailayangiri S, Altvater B, Lesch S, Balbach S, Göttlich C, Kühnemundt J, Mikesch J-H, Schelhaas S, Jamitzky S, Meltzer J, Farwick N, Greune L, Fluegge M, Kerl K, Lode HN, Siebert N, Müller I, Walles H, Hartmann W, Rössig C
  (See online at https://doi.org/10.1016/j.ymthe.2019.02.014)
• Introducing Specificity to Iron Oxide Nanoparticle Imaging by Combining (57)Fe-Based MRI and Mass Spectrometry. Nano Lett. 2019
  Masthoff M, Buchholz R, Beuker A, Wachsmuth L, Kraupner A, Albers F, Freppon F, Helfen A, Gerwing M, Holtke C, Hansen U, Rehkamper J, Vielhaber T, Heindel W, Eisenblatter M, Karst U, Wildgruber M, Faber C
  (See online at https://doi.org/10.1021/acs.nanolett.9b03016)
• Loss of the transcription factor RBPJ induces disease-promoting properties in brain pericytes. Nat Commun. 2019; 10: 2817
  Dieguez-Hurtado R, Kato K, Giaimo BD, Nieminen-Kelha M, Arf H, Ferrante F, Bartkuhn M, Zimmermann T, Bixel MG, Eilken HM, Adams S, Borggrefe T, Vajkoczy P, Adams RH
  (See online at https://doi.org/10.1038/s41467-019-10643-w)
• Mechanochemical self-organization determines search pattern in migratory cells. Nat Phys. 2019; 15: 848-857
  Begemann I, Saha T, Lamparter L, Rathmann I, Grill D, Golbach L, Rasch C, Keller U, Trappmann B, Matis M, Gerke V, Klingauf J, Galic M
  (See online at https://doi.org/10.1038/s41567-019-0505-9)
• Polarization nano-tomography of tightly focused light landscapes by self-assembled monolayers. Nat Commun. 2019; 10: 4308
  Otte E, Tekce K, Lamping S, Ravoo BJ, Denz C
  (See online at https://doi.org/10.1038/s41467-019-12127-3)
• Portable all-fiber dual-output widely tunable light source for coherent Raman imaging. Biomed Opt Express. 2019; 10: 4437-4449
  Brinkmann M, Fast A, Hellwig T, Pence I, Evans CL, Fallnich C
  (See online at https://doi.org/10.1364/BOE.10.004437)
• Rab11 is required for neurite pruning and developmental membrane protein degradation in Drosophila sensory neurons. Dev Biol. 2019; 451: 68-78
  Krämer R, Rode S, Rumpf S
  (See online at https://doi.org/10.1016/j.ydbio.2019.03.003)
• Stereospecific alpha-Sialylation by Site-Selective Fluorination. Angew Chem Int Ed Engl. 2019; 58: 3814-3818
  Hayashi T, Kehr G, Bergander K, Gilmour R
  (See online at https://doi.org/10.1002/anie.201812963)