Struktur und Orientierung von Kollagen und Lipidmolekülen auf Titanoxideoberflächen: eine spektroelektrochemische Untersuchung
Final Report Abstract
Despite numerous complications the aims of the project have been achieved. The final analysis concerning the morphology of collagen films were done. Studies of new substrate: titanium oxide carbide have been continued and provided new, unexpected and interesting data concerning the complex process of the collagen adsorption. The number of publications concerning studies of collagen structure, reactivity as well as the application of collagen in various segments of industry is very high. This is an interesting protein, which is the most abundant in human body, which structure is unique among all proteins and which has diverse functions in organisms. Therefore, it wakes a large scientific interest. Despite these facts, the structure, dynamics of structural and conformational changes in the protein, its reactivity and process adsorption on various surfaces are not well known. Results of these studies gave a first experimental evidence of conformational changes in the structure of the collagen helix in the adsorbed state. They confirmed that the presence of water is crucial for the maintenance of the native collagen structure. In the presence of water the helical structure of collagen assemblies is very stable. Changes in the protein structure were observed neither under the presence of high electric fields nor at high temperatures. Infrared spectroscopy in very often used to analyse the secondary structure of proteins. The analysis of IR spectra of various collagen films let us to revise the assignment of amide I modes to different structural units and hydrogen bonds along the helix. These results agree exactly with the recent x-ray diffraction data concerning the structure of collagen. The results of this project provide new information concerning pre-implantation modification of implant surfaces by collagen films.
Publications
- J. Electroanal. Chem., 706, 2013, 140- 148. “Study of the potential driven changes in a collagen film self-assembled on a polycrystalline gold electrode surface”
Ahlers, M.; Stein, N.; Broch, L.; Brand, I.
(See online at https://doi.org/10.1016/j.jelechem.2013.08.010) - “Application of infrared spectroscopy for structural analysis of planar lipid bilayers under electrochemical control” in “Advances in Planar Lipid Bilayers and Liposomes“, Eds. A. Iglic and C.V. Kulkarni, Academic Press, Vol. 18, 2013, 21-62
Brand, I.
(See online at https://doi.org/10.1016/B978-0-12-411515-6.00002-3) - ”Electrocemical and spectroscopic analysis of collagenfilms and lipid bilayers on biomaterial surfaces”
M. Ahlers
- 2014, Shaker Verlag, Aachen, ISBN:978-3-8440-2965-9, pp.121 „Application of infrared spectroscopy for structural analysis of thin molecualr films under electrochemical control“
Izabella Brand
- Coll. Surf. B, 116, 2014, 389-395. “A Langmuir monolayer study of the action of phospholipase A2 on model phospholipid and mixed phospholipid-GM1 ganglioside membranes”
Schulte, W.; Orlof, M.; Brand, I.; Krochowiec, B.; Rogalska, E.
(See online at https://doi.org/10.1016/j.colsurfb.2013.12.032) - Surface Science, special issue Electrochemical Surface Science, 2014, “Impact of temperature and electrical potentials on the stability and structure of collagen adsorbed on the gold electrode”
Meiners, F.; Ahlers, M.; Brand I., Wittstock, G.
(See online at https://doi.org/10.1016/j.susc.2014.07.001)
