Project Details
Projekt
Soluble carbonic anhydrase IX and its potential role as salivary tumor marker in renal carcinoma
Applicant
Dr. Marcus Mau
Subject Area
Veterinary Medical Science
Term
from 2010 to 2013
Project identifier
Deutsche Forschungsgemeinschaft (DFG) - Project number 175653553
The use of saliva for detection of oral diseases is well confirmed, whereas its value to identify systemic diseases is rather unclear. There are reports about the presence of biomarkers of systemic cancers in saliva. However, the marker origin and the underlying mechanisms of the secretion into saliva remain unsolved. In this project, a mouse RENCA tumor model will be used to develop renal tumors remote from the oral cavity. Salivary proteome and transcriptome will be analyzed for biomarker profiles with primary focus on carbonic anhydrase IX (CA-IX), which is overexpressed and secreted to the blood by renal carcinoma. A preliminary immunoblot experiment revealed that CA-IX might be present in some human salivary secretions. There are indications that salivary markers directly originate from tumors or are induced in salivary glands by tumor-produced factors like nerve growth factor (NGF). To learn more about the origin and fate of CA-IX, it is intended to use CA-IX-GFP-transfected RENCA cells in the experiments. Thereby, the protein can be easily followed from the tumor into blood and possibly saliva. This is the first attempt to put some light on the origin and secretion of biomarkers of systemic cancer in saliva.
DFG Programme
Research Fellowships
International Connection
United Kingdom, USA
