Quantifying diffusion and dynamics of lipids within membranes in a leaflet-dependent manner (A10)

• Subject Area: Biophysics
• Term: from 2010 to 2020
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 55908123

The goal of this project is to study lipid bilayers with fluorescence spectroscopy that can distinguish between the two leaflets of a bilayer. In particular, we want to understand lipid diffusion in a leaflet-specific manner, lipid exchange dynamics between leaflets, and membrane fusion. The core method that will be used is our recently developed Metal-Induced Energy Transfer (MIET) microscopy, which exploits the extremely sensitive dependence of metal-induced fluorescence quenching on the distance between a metal surface and the quenched emitter.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 803: Functionality Controlled by Organisation in and between Membranes
• Major Instrumentation: Scanner Unit
• Instrumentation Group: 5080 Optisches Mikroskopzubehör
• Applicant Institution: Georg-August-Universität Göttingen
• Project Head: Professor Dr. Jörg Enderlein