Ribosome dynamics in translation (A03)

• Subject Area: Biochemistry
• Term: from 2010 to 2022
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 105286809

During protein synthesis, ribosome, tRNAs, and protein factors move in a precisely orchestrated way to translate mRNA into a functional polypeptide. We will use biophysical techniques (ensemble and single molecule fluorescence and FRET) in combination with structural analysis to investigate the conformational and compositional dynamics of the ribosome-tRNA-translation factor complexes at each step of translation. We will analyse and how ribosome dynamics change in response to translation pausing and recoding events. This work will reveal how spontaneous Brownian motions of molecules are translated into biological function.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 860: Integrative Structural Biology of Dynamic Macromolecular Assemblies
• Applicant Institution: Georg-August-Universität Göttingen
• Project Head: Professorin Dr. Marina V. Rodnina