The sub-module for spliceosome catalytic activation (A04)

• Subject Area: Structural Biology
• Term: from 2010 to 2018
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 25065445

Using integrative structural biochemistry, project A04 will investigate the architecture of a large spliceosomal submodule, which consists of the Brr2 RNA helicase, the Snu114 G-protein and the Prp8 regulatory scaffold and which is required for RNP remodeling events that elicit spliceosome catalytic activation, as well as of its substrate complex. We will then analyze how molecular, submodular and modular functions of the spliceosome are affected by structure-guided site-directed mutagenesis and will also investigate how the submodule is regulated by reversible ubiquitination.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 740: From Molecules to Modules: Organisation and Dynamics of Functional Units in Cells
• Major Instrumentation: Multi-angle static laser light scattering unit
• Instrumentation Group: 1350 Flüssigkeits-Chromatographen (außer Aminosäureanalysatoren 317), Ionenaustauscher
• Applicant Institution: shared FU Berlin and HU Berlin through:; Charité - Universitätsmedizin Berlin
• Co-Applicant Institution: Freie Universität Berlin
• Project Head: Professor Dr. Markus C. Wahl