Project Details
Projekt
Lipid composition of human immunodeficiency virus type 1 and its relevance for particle formation, stability and infectivity (14)
Subject Area
Virology
Term
from 2010 to 2021
Project identifier
Deutsche Forschungsgemeinschaft (DFG) - Project number 112927078
HIV-1 budding occurs at the plasma membrane and is achieved by assembly of the viral Gag polyprotein. In this project, we study protein-lipid interactions at HIV-1 assembly sites to determine mechanism and function of virus-specific microdomain formation. We will apply live-cell and super-resolution imaging of proteins and lipids at viral assembly sites, using synchronized plasma membrane recruitment of Gag and clickable lipids. This will be extended by correlative light and electron microscopy and tomography of (induced) assembly sites. We will furthermore extend our lipidomic and lipid-protein cross-linking studies and plan to move towards in situ mass spectrometry to determine lipids directly at the viral budding site of intact cells.
DFG Programme
CRC/Transregios
Applicant Institution
Ruprecht-Karls-Universität Heidelberg
Project Head
Professor Dr. Hans-Georg Kräusslich
