Molekulare Struktur und Assemblierung von Cniden (Nematocysten)

• Applicant: Professor Dr. Thomas W. Holstein
• Subject Area: Evolutionary Cell and Developmental Biology (Zoology)
• Term: from 2006 to 2011
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 15725069

• Final Report Year: 2012

Final Report Abstract

No abstract available

Publications

• Nanosecond-scale kinetics of nematocyst discharge. Curr Biol. 2006; 16(9): R316-8
  Nüchter T, Benoit M, Engel U, Özbek S, Holstein TW
  
• Structure/function analysis of spinalin, a spine protein of Hydra nematocysts. FEBS J. 2006; 273(14): 3230-7
  Hellstern S, Stetefeld J, Fauser C, Lustig A, Engel J, Holstein TW, Özbek S
  
• A biological cosmos of parallel universes: does protein structural plasticity facilitate evolution? Bioessays. 2007; 29(11): 1095-104
  Meier S, Özbek S
  
• Continuous molecular evolution of protein-domain structures by single amino acid changes. Curr Biol. 2007; 17(2): 173-8
  Meier S, Jensen PR, David CN, Chapman J, Holstein TW, Grzesiek S, Özbek S
  
• Sequence-structure and structure-function analysis in cysteine-rich domains forming the ultrastable nematocyst wall. J Mol Biol. 2007; 368(3): 718-28
  Meier S, Jensen PR, Adamczyk P, Bächinger HP, Holstein TW, Engel J, Özbek S, Grzesiek S
  
• Evolution of complex structures: minicollagens shape the cnidarian nematocyst. Trends Genet. 2008; 24(9): 431-8
  David CN, Özbek S, Adamczyk P, Meier S, Pauly B, Chapman J, Hwang JS, Gojobori T, Holstein TW
  
• Minicollagen-15, a novel minicollagen isolated from Hydra, forms tubule structures in nematocysts. J Mol Biol. 2008; 376(4):1008-20
  Adamczyk P, Meier S, Gross T, Hobmayer B, Grzesiek S, Bächinger HP, Holstein TW, Özbek S
  
• Cnidarians and the evolutionary origin of the nervous system. Dev Growth Differ. 2009; 51(3): 167-83
  Watanabe H, Fujisawa T, Holstein TW
  
• Cnidocyst structure and the biomechanics of discharge. Toxicon. 2009; 54(8): 1038-45
  Özbek S, Balasubramanian PG, Holstein TW
  
• (2009). The Dynamic Genome of Hydra. Nature. 2010; 464(7288): 592-6
  Chapman JA, Kirkness EF, Simakov O, Hampson SE, Mitros T, Weinmaier T, Rattei T, Balasubramanian PG, Borman J, Busam D, Disbennett K, Sumin N, Sutton GG, Viswanathan LD, Walenz B, Goodstein DM, Hellsten U, Kawashima T, Prochnik SE, Putnam NH, Shu S, Blumberg B, Dana CE, Gee L, Kibler DF, Law L, Lindgens D, Martinez DE, Peng J, Wigge PA, Bertulat B, Guder C, Nakamura Y, Özbek S, Watanabe H, Khalturin K, Hemmrich G, Franke A, Augustin R, Fraune S, Hayakawa E, Hayakawa S, Hirose M, Hwang JS, Ikeo K, Nishimiya- Fujisawa C, Ogura A, Takahashi T, Steinmetz PRH, Zhang X, Aufschnaiter R, Eder MK, Gorny AK, Salvenmoser W, Heimberg AM, Wheeler BM, Peterson KJ, Böttger A, Tischler P, Wolf A, Gojobori T, Remington KA, Strausberg RL, Venter JC, Technau U, Hobmayer B, Bosch TCG, Holstein TW, Fujisawa T, Bode HR, David CN, Rokhsar DS, Steele RE
  
• A non-sulfated chondroitin stabilizes membrane tubulation in cnidarian organelles. J Biol Chem. 2010; 285(33): 25613-23
  Adamczyk P, Zenkert C, Balasubramanian PG, Yamada S, Murakoshi S, Sugahara K, Hwang JS, Gojobori T, Holstein TW, Özbek S
  
• Nematogalectin, a nematocyst protein with GlyXY and galectin domains, demonstrates nematocyte-specific alternative splicing in Hydra. Proc Natl Acad Sci U S A. 2010; 107(43): 18539-44
  Hwang JS, Takaku Y, Momose T, Adamczyk P, Özbek S, Ikeo K, Khalturin K, Hemmrich G, Bosch TC, Holstein TW, David CN, Gojobori T
  
• Preparation techniques for transmission electron microscopy of Hydra. Methods Cell Biol. 2010; 96: 285-306
  Holstein TW, Hess MW, Salvenmoser W
  
• The evolution of extracellular matrix. Mol Biol Cell. 2010; 21(24): 4300-5
  Özbek S, Balasubramanian PG, Chiquet-Ehrismann R, Tucker RP, Adams JC
  
• Morphological and molecular analysis of the Nematostella vectensis cnidom. PLoS One. 2011; 6(7): e22725
  Zenkert C, Takahashi T, Diesner MO, Özbek S
  
• The cnidarian nematocyst: a miniature extracellular matrix within a secretory vesicle. Protoplasma. 2011; 248(4): 635-40
  Özbek S
  
• A view to kill. BMC Biol. 2012 Mar 5;10:18
  Holstein TW
  
• Neurotoxin localization to ectodermal gland cells uncovers an alternative mechanism of venom delivery in sea anemones. Proc Biol Sci. 2012; 279(1732): 1351-8
  Moran Y, Genikhovich G, Gordon D, Wienkoop S, Zenkert C, Özbek S, Technau U, Gurevitz M
  (See online at https://doi.org/10.1098/rspb.2011.1731)
• Proteome of Hydra nematocyst. J Biol Chem. 2012; 287(13): 9672-81
  Balasubramanian PG, Beckmann A, Warnken U, Schnölzer M, Schüler A, Bornberg-Bauer E, Holstein TW, Özbek S
  
• The nematocyst: a molecular map of the cnidarian stinging organelle. Int J Dev Biol. 2012; 56(6-8): 577-82
  Beckmann A, Özbek S
  (See online at https://doi.org/10.1387/ijdb.113472ab)