Project Details
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Regulation of osmolyte synthesis in the moderate halophile Halobacillus halophilus

Subject Area Metabolism, Biochemistry and Genetics of Microorganisms
Term from 2009 to 2013
Project identifier Deutsche Forschungsgemeinschaft (DFG) - Project number 150413131
 
Final Report Year 2014

Final Report Abstract

Although we did not get to far with respect to solving the most interesting question: what is the molecular basis of the chloride dependence of H. halophilus, we feel that we were quite successfull and have accomplished to publish a number of papers on different aspects on the physiology of H. halophilus. Our initial hypothesis that the glutamine synthetase GlnA2 senses and responds to chloride had to be revisited. It took us some time and the development of a genetic system to realize that. However, our initial observation that a glutamytransferase activity is salt-dependent and chloride stimulated is still valid and has been reproduced by several students. Using the genetic system we were then able to screen for other enzymes that catalyze the salt-dependent glutamytransferase activity as observed in whole cells but the potential candidates analzyed so far turned out to be the false ones. Currently, this is the search for the needle in the haystack and, therefore, we currently refrain from continuation of the project.

Publications

 
 

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