Consequences of chronic hypoxia and HIF-1/HIF-2 in regulating function and plastic-ity of macrophages and tumor cells (A08)

• Subject Area: Public Health, Healthcare Research, Social and Occupational Medicine ; Cell Biology
• Term: from 2009 to 2020
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 61867463

The role of chronic hypoxia in affecting biological systems is rarely known, although affecting expression of genes via tristetraprolin. We determine the post-transcriptional regulation of genes via tristetraprolin using the individual-nucleotide resolution UV cross-linking and immunoprecipitation- and NGS-method, to understand the role of affected genes in macrophages. In addition, HIF-1 impairs the assembly of complex I of the mitochondrial respiratory chain by inducing β-TrCP (β-transducin repeat-containing protein) and degrading the assembly factor TMEM126B. The relevance of this observation towards the functional role of mitochondria in determining macrophages and tumor cells will be evaluated in vitro and in vivo.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 815: Redox-Regulation: Generating Systems and Functional Consequences
• Applicant Institution: Goethe-Universität Frankfurt am Main
• Project Head: Professor Dr. Bernhard Brüne